摘要
NAC转录因子参与植物对生物和非生物胁迫响应过程。本研究以甘蔗野生种割手密为材料,克隆得到3个SsNACs基因,命名SsNAC2、SsNAC3、SsNAC4。生物信息学分析表明,其编码序列CDS全长931 bp、486 bp、781 bp,编码309个、162个、259个氨基酸。亚细胞定位预测3个SsNACs基因位于细胞核,上游启动子含有与逆境胁迫应激相关的ABRE、LTR、MBS、MYB、STRE顺式作用元件。系统进化分析表明SsNAC2与高粱SbNAC68亲缘关系最近,属于OsNAC3亚家族,SsNAC3与南荻MlNAC1亲缘关系最近,属于ATAF亚家族,SsNAC4与高粱SbNAC82同属NAC2亚家族。组织特异性表达分析结果SsNAC2、SsNAC3基因在割手密茎叶生长期表达量较高。基因表达谱分析表明,不同胁迫下3个SsNACs基因的相对表达水平不同,推测SsNAC2、SsNAC3和SsNAC4基因可能参与调控割手密应对干旱、低温、盐分、病原真菌等非生物和生物胁迫,同时又能够被ABA和MeJA诱导表达,以上研究为进一步分析NAC转录因子在割手密响应逆境胁迫中的功能奠定理论基础。
NAC transcription factors are known to be involved into responses to biotic and abiotic stresses in plants.In this study,we cloned by sequence homology 3 SsNAC genes from sugarcane wild species S.spontaneum L.,named as SsNAC2,SsNAC3 and SsNAC4.Bioinformatic analysis showed that the full-length coding sequences of SsNAC2,SsNAC3 and SsNAC4 were 931 bp,486 bp,and 781 bp,encoding 309,162 and 259 amino acids,respectively.The subcellular location prediction indicated that the deduced proteins of SsNAC2,SsNAC3 and SsNAC4 were localized in the nucleus.The promoters of SsNAC2,SsNAC3 and SsNAC4 were predicted with putative stress-responsive cis-acting elements including an ABRE,LTR,MBS,MYB and STRE.Phylogenetic tree analysis showed that SsNAC2 within the OsNAC3 subgroup was closely related to Sorghum SbNAC68 gene.SsNAC4 and its homolog gene sorghum SbNAC82 belonged to NAC2 subgroup,and SsNAC3 in the ATAF subgroup was closely related to Miscanthus MlNAC1.The tissue-specific analysis showed that SsNAC2 and SsNAC3 were highly expressed in stem and leaves.The transcription profile analysis showed that SsNAC2,SsNAC3 and SsNAC4 were variable on expression under different stress conditions.It′s speculated that SsNAC2,SsNAC3 and SsNAC4 might be involved in regulation of abiotic and biotic stresses including drought,low temperature,salinity,pathogenic fungi,etc.The transcripts of SsNAC2,SsNAC3 andSsNAC4 were inducible by ABA and MeJA treatments.These results provided theoretical basis for future deciphering the functions of NAC genes under stresses conditions in S.Spontaneum L.
作者
沈庆庆
钱禛锋
谷书杰
吕绍芝
赵雪婷
何丽莲
李富生
SHEN Qing-qing;QIAN Zhen-feng;GU Shu-jie;LYU Shao-zhi;ZHAO Xue-ting;HE Li-lian;LI Fu-sheng(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201;Sugarcane Research Institute,Yunnan Agricultural University,Kunming 650201;The Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province,Kunming 650201)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第3期906-916,共11页
Journal of Plant Genetic Resources
基金
国家重点研发计划项目(2018YFD1000503)
国家自然科学基金(31960451)
云南省作物生产与智慧农业重点实验室专项(202105AG070007)
云南省教育厅科学研究基金项目(2020Y203)。
关键词
割手密
NAC转录因子
基因克隆
表达分析
Saccharum spontaneum L.
NAC transcription factor
gene cloning
expression analysis
