摘要
为了解植物miR171家族成员的进化特性及在文心兰侵染软腐病过程中的表达模式,运用生物信息学的方法,从miRbase数据库、文心兰转录组数据库中获取miR171家族48个物种268个前体序列和326个成熟体序列,进行植物学分类、构建进化树、预测二级结构、靶基因预测及实时荧光定量等手段,对文心兰miR171基因家族进行研究.生信分析结果显示:(1)植物miR171家族分布十分广泛,在不同物种间数量分布差异显著,苔藓植物可能是miR171家族进化的祖先,在禾本科植物中进化较完全;(2)系统进化树显示,物种的进化与植物miR171家族成员进化有一定的关系;(3)二级结构预测表明植物miR171家族成员均能形成典型的茎环结构,整体保守性较高,其最小折叠自由能在不同植物中差异显著.为验证以上推论,在文心兰侵染软腐病过程中分析miR171和其靶基因的表达量,结果表明pre-miR171在假鳞茎的相对表达量最高,是根相对表达量的12倍,在叶和根中表达量逐渐减少,且在假鳞茎中随着处理时间的延长,premiR171表达量先上升后下降,而靶基因的表达呈现出与之相反的趋势.本研究表明植物miR171家族成员表达具有时空特异性,且主要靶向GRAS基因家族发挥功能.(图8表3参40)
This study sought to understand the evolutionary characteristics of miR171 family members and their expression patterns during soft rot infection in Oncidium hybridum. Based on the miRbase and the transcriptome, and genome databases of O. hybridum, 268 precursors and 326 mature sequences of the miR171 family in 48 plant species were analyzed using distribution statistics, construction of phylogenetic trees,prediction of secondary structures, prediction of target genes, and detection of their expression levels by realtime fluorescence quantification. The results of the biometric analysis showed that the miR171 family was widely distributed in the plant community, and bryophytes were the evolutionary ancestors of the miR171 family. In addition, the miR171 family has evolved relatively completely in grasses. The phylogenetic tree showed that the evolution of species has a certain relationship with the evolution of the miR171 family members. Mfold predicted that pre-miR171 spontaneously forms a typical, stable stem-loop secondary structure, with relatively high conservation. The minimum free radical energy varies significantly among different plants. To verify this inference, we analyzed the expression of miR171 and its target genes during soft rot infection in O. hybridum.The relative expression of pre-miR171 in pseudobulbs was highest, being 12 times the relative expression in roots. The expression in leaves and roots gradually decreased. Moreover, in pseudobulbs, with the prolongation of the treatment time, the expression of pre-miR171 first increased and then decreased. The expression of target genes showed the opposite trend. The expression of miR171 family members has temporal and spatial specificity and mainly targets the GRAS gene family to function.
作者
吴秋桢
林争春
倪珊珊
张舒婷
张梓浩
林玉玲
赖钟雄
WU Qiuzhen;LIN Zhengchun;NI Shanshan;ZHANG Shutin;ZHANG Zihao;LIN Yuling;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2022年第2期498-507,共10页
Chinese Journal of Applied and Environmental Biology
基金
福建省重大农业科技专项(2015NZ0002-1)
福建农林大学科技创新基金项目(KF2015108,CXZX2018076)资助。
关键词
miR171家族
进化规律
分子特性
文心兰
软腐病
miR171 family
evolutionary regularity
molecular characteristics
Oncidium
soft rot
