摘要
为探讨不同粒径生物质炭对土壤重金属钝化及细菌群落的影响。本研究对海鲜菇和秀珍菇废菌棒生物质炭进行不同粒径(粗:1—2 mm、中0.5—1 mm、细<0.5 mm)处理,向污染矿山土壤中分别加入质量分数为0%和2%不同粒径生物质炭,恒温培养120 d,分析不同处理对土壤pH、速效养分、有效态重金属、酶活性、细菌群落结构和多样性的影响。结果显示,与对照相比,不同粒径生物质炭处理均能提高土壤pH、速效氮、有效磷和有效钾含量,增强了脲酶、蛋白酶、转化酶活性,提升了土壤细菌群落Chao1和ACE指数,并提高了Kribbella、Streptomyces、Paenarthrobacter、Micromonospora、Bradyrhizobium等16个优势有益菌属的相对丰度,且以上指标均以细粒径生物质炭的提升效果为最佳。不同粒径生物质炭有效降低TCLP提取态Cd(12.1%—24.8%)、Zn(8.3%—31.4%)、Pb(32.9%—53.7%)的质量分数,且粒径越小钝化作用越好。综上,废菌棒生物质炭可以有效改良土壤酸性,降低有效态重金属,提高土壤速效养分和土壤酶活性,提高细菌多样性和有益菌群,且细粒径生物质炭的改良效果优于粗粒径。研究结果可为重金属污染土壤的修复改良提供理论支持。
This study investigated the effects of biochar with different particle sizes on soil heavy metal immobilization and the bacterial community.Different particle sizes(1‒2 mm in diameter,0.5‒1 mm in diameter,and<0.5 mm in diameter)of biochar from P.marmoreus and P.crispus were chosen.Biochar with different mass fractions,0%and 2%respectively,were added to the contaminated mine soil,which was incubated at a constant temperature for 120 days.The effects of different treatments on soil pH,available nutrients,available heavy metals,enzyme activities,and bacterial community structure and diversity were analyzed.The results showed that biochar treatments with different particle sizes could increase the soil pH,available nitrogen,available phosphorus,and available potassium content,as well as enhance the activities of urease,protease,and invertase,but similar effects did not occur in the control group.For the bacterial community,the biochar treatment increased the Chao1 and ACE indexes of soil bacterial community,and enhanced relative abundance of 16 dominant beneficial bacteria such as Kribbella,Streptomyces,Paenarthrobacter,Micromonospora,and Bradyrhizobium.Fine particle size biochar(<0.5 mm in diameter)showed the best improvement effects on the soil bacterial community.The contents of Cd(12.1%‒24.8%),Zn(8.3%‒31.4%)and Pb(32.9%‒53.7%)extracted by TCLP were effectively reduced by biochar with different particle sizes,and the smaller the particle size,the better the immobilization effect.In conclusion,biochar can effectively improve soil acidity,reduce available heavy metals,increase soil available nutrients and soil enzyme activity, and improve bacterial diversity.
作者
李光炫
石岸
张黎明
邢世和
杨文浩
LI Guangxuan;SHI An;ZHANG Liming;XING Shihe;YANG Wenhao(College of Resources and Environmental,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China;University Key Lab of Soil Eeosyslem Health and Regulation,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China)
出处
《生态环境学报》
CSCD
北大核心
2022年第3期583-592,共10页
Ecology and Environmental Sciences
基金
福建省自然科学基金项目(2020J01565)
福建农林大学科技创新基金项目(KFA19075A
KFA19076A)。
关键词
生物质炭
粒径
土壤
重金属钝化
细菌群落
biocar
particle size
soil,heavy metal immobilization
bacterial community
