摘要
为研究牛至精油对绵羊瘤胃体外发酵特性及CH_(4)产量的影响,采用单因素完全随机试验设计,利用体外法,在培养液中分别添加0(CON)、0.5%(OEO1)、1.0%(OEO2)、1.5%(OEO3)的牛至精油,每组3个重复。在人工瘤胃缓冲液中发酵48 h后测定挥发性脂肪酸及不同时间总产气量和CH_(4)产量。结果表明,发酵2、24、48 h时,总产气量、CH_(4)产量随牛至精油添加量的增加呈显著二次曲线变化(P<0.05)。与CON组相比,发酵2 h和48 h时,OEO3组总产气量、CH_(4)产量显著降低(P<0.05),OEO1组和OEO2组总产气量无显著差异(P>0.05);从CH_(4)产量比例来看,发酵24 h和48 h时,随牛至精油添加量的增加甲烷比例呈极显著二次曲线变化(P<0.01),与CON组相比,发酵24 h和48 h时,OEO3组CH_(4)比例显著降低(P<0.05);与CON组相比,OEO3组TVFA浓度、丁酸比例显著降低(P<0.05)。综上所述,牛至精油能够降低瘤胃体外发酵的总产气量和CH_(4)产量,改变CH_(4)产量比例,调控瘤胃发酵。
This study aimed to observe the effects of oregano essential oil on rumen fermentation and CH_(4)production of sheep in vitro.A single-factor experimental design was used in this study.Oregano essential oil was supplemented in culture medium for different groups at levels of 0(CON),0.5%(OEO1),1%(OEO2),1.5%(OEO3),respectively.Each group had 3 replicates.After fermented for 48 h,fermentation parameters,total gas and CH_(4)production were measured.The results showed as follows:the total gas production and CH_(4)production showed significant quadratic curve changes with the increase of oregano essential oil supplemental level at 2,24,48 h(P<0.05);compared with the CON group,after fermentation for 2 h and 48 h,the total gas production and CH_(4)production in OEO3 group were significantly decreased(P<0.05);in terms of the proportion of CH_(4)production,the proportion of methane showed a significant quadratic curve change with the increase of oregano essential oil content at 24 h and 48 h of fermentation(P<0.01);compared with the CON group,the proportion of CH_(4)production was significantly decreased at 24 h and 48 h of fermentation(P<0.05);TVFA concentration and butyrate proportion in OEO3 group were significantly decreased compared with CON group(P<0.05).In conclusion,oregano essential oil can reduce total gas production and CH_(4)production,change the ratio of CH_(4)production,and regulate rumen fermentation.
作者
刘立山
周瑞
王彩莲
郎侠
宋淑珍
LIU Lishan;ZHOU Rui;WANG Cailian;LANG Xia;SONG Shuzhen(Gansu Academy of Agricultural Sciences,Animal Husbandry,Pasture and Green Agriculture Institute,Key Laboratory for Sheep,Goat,and Cattle Germplasm and Straw Feed in Gansu Province,Lanzhou 730070,China;Life Science and Engineering College of Northwest University for Nationalities,Lanzhou 730070,China)
出处
《中国草食动物科学》
CAS
2022年第3期38-41,50,共5页
China Herbivore Science
基金
甘肃省青年科技基金计划(20JR5RA103)
西北民族大学人才引进项目(Z20066)
国家民委专项(31929210004)
甘肃省青年科技基金计划(21JR1RA213)。
关键词
牛至精油
体外发酵
发酵特性
CH_(4)产量
oregano essential oil
in vitro fermentation
fermentation characteristics
CH_(4)production
