摘要
目的探索结核分枝杆菌感染及常规抗结核化疗过程中肠道菌群的变化差异,为结核病的临床治疗提供科学依据。方法提取89份粪便样本的基因组DNA,利用宏基因组测序技术及生物信息学方法分析结核分枝杆菌感染及常规抗结核化疗过程中肠道微生物菌群的变化。结果结核分枝杆菌感染后,结核病患者的肠道菌群结构组成发生显著改变(P=0.0134)。随着常规抗结核化疗时间延长,Shannon指数下降,结核病患者肠道菌群多样性明显降低(P=0.0088),结核病患者治愈后菌群结构组成较治疗前出现显著变化(P=0.0021)。结核分枝杆菌感染及常规抗结核化疗过程中,拟杆菌属和韦永氏球菌属的相对丰度逐渐增加。经抗结核化疗后结核病患者肠道内微生物群中部分功能途径发生变化。结论结核分枝杆菌感染及常规抗结核化疗可以导致肠道菌群紊乱,对肠道菌群的多样性、结构组成和代谢功能等产生影响。
Objective To explore the changes of intestinal flora during Mycobacterium tuberculosis infection and conventional anti-tuberculosis chemotherapy,and to provide scientific basis for clinical treatment of tuberculosis.Methods Genomic DNA was extracted from 89 stool samples,and the changes of intestinal microflora during mycobacterium tuberculosis infection and conventional anti-tuberculosis chemotherapy were analyzed by metagenomic sequencing and bioinformatics methods.Results After mycobacterium tuberculosis infection,the composition of intestinal microflora in tuberculosis patients was significantly changed(P=0.0134).With the prolongation of conventional anti-tuberculosis chemotherapy,the Shannon index decreased,and the diversity of intestinal flora in tuberculosis patients was significantly reduced(P=0.0088),and the composition of bacterial flora in tuberculosis patients after cure was significantly changed compared with that before treatment(P=0.0021).The relative abundances of Bacteroides and Veillonella increased gradually during Mycobacterium tuberculosis infection and routine anti-tuberculosis chemotherapy.Some functional pathways in the gut microbiota of tuberculosis patients were changed after anti-tuberculosis chemotherapy.Conclusion Mycobacterium tuberculosisinfection and conventional anti-tuberculosis chemotherapy can lead to disturbance of intestinal flora,affecting the diversity,structural composition and metabolic function of intestinal flora.
作者
张晨晨
余美玲
谭卫国
巫株华
魏文静
ZHANG Chenchen;YU Meiling;TAN Weiguo;WU Zhuhua;WEI Wenjing(Research Institute of Tuberculosis,Centre for Tuberculosis Control of Guangdong Province,Guangzhou 510630,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2022年第6期678-684,共7页
The Journal of Practical Medicine
基金
“十三五”国家科技重大专项(编号:2018ZX10715004-002-021)
广东省医学科学技术研究基金(编号:A2020338,B2021012)
佛山市第四人民医院“登峰计划”高水平医院建设项目(编号:FSSYKF-2020015)。
关键词
结核分枝杆菌
肠道菌群
抗结核药物
Mycobacterium tuberculosis
intestinal flora
anti-tuberculosis drugs
