摘要
目的探讨miR-581对卵巢癌SKOV3细胞自噬的影响及作用机制。方法将miR-581 mimics和miR-581 NC转染到卵巢癌SKOV3细胞中,实时荧光定量PCR法(qRT-PCR)检测转染效率;转染成功后,Western blot检测转染后卵巢癌SKOV3细胞中自噬相关蛋白的表达水平;TargetScanHuman数据库预测miR-581靶基因,Western blot验证miR-581与靶基因的作用。结果过表达miR-581后可显著抑制自噬相关蛋白LC3Ⅱ和Beclin1的表达(P<0.01);miR-581可负向调控FOXO1的表达(P<0.01);FOXO1促进卵巢癌SKOV3细胞自噬(P<0.05)。结论miR-581通过调控FOXO1的表达影响卵巢癌SKOV3细胞自噬。
Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells.Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells,and the transfection efficiency was detected by qRT-PCR.After successful transfection,Western blot was used to detect autophagy-related proteins expression in SKOV3 cells.TargetScanHuman database predicted miR-581 target genes,and Western blot verified the role of miR-581 and target genes.Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3Ⅱand Beclin1(P<0.01);miR-581 negatively regulated FOXO1 expression(P<0.01)and FOXO1 promoted autophagy of SKOV3 cells(P<0.01).Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.
作者
高海宁
白瑞霞
赵鹏伟
宋婉莹
林萱
GAO Haining;BAI Ruixia;ZHAO Pengwei;SONG Wanying;LIN Xuan(Inner Mongolia Medical College,Inner Mongolia Medical University,Hohhot 010000,China;Department of Clinical Laboratory,Inner Mongolia People’s Hospital,Hohhot 010000,China;Graduate School,Inner Mongolia Medical University,Hohhot 010000,China;Department of Clinical Laboratory,Xing'anmeng People's Hospital,Xing'anmeng 137400,China)
出处
《肿瘤防治研究》
CAS
CSCD
2022年第5期403-407,共5页
Cancer Research on Prevention and Treatment
