摘要
目的探究2型糖尿病人与正常健康人血浆外泌体中蛋白质组的差异,为2型糖尿病早期诊断、预后判断和寻找治疗标靶提供科学依据。方法收集6例确诊2型糖尿病患者与6例同期健康人血浆,用IZON qEV高纯度外泌体分离柱分离外泌体,提取蛋白,经液相色谱质谱联用鉴定分析2型糖尿病人与正常健康人血浆外泌体蛋白表达的差异,并进行生物信息学分析。结果共检测到可定量的血浆外泌体蛋白质948个。与正常健康人相比,其中23个在2型糖尿病人血浆外泌体中表达上调,124个表达下调;上调蛋白质中很多蛋白分子,包括补体成分,与炎症下免疫反应相关。结论2型糖尿病人与正常健康人血浆外泌体蛋白有一定的表达差异,这种外泌体蛋白组分差异有可能为2型糖尿病早期诊断提供新的候选分子并为其阐明发病机理提供证据。
Objective To explore the difference in plasma exosomal proteome between type 2 diabetes mellitus(T2 DM) patients and healthy subjects, and to provide a scientific basis for early diagnosis, prognosis judgment and treatment targets. Methods Blood plasma samples were collected from 6 T2DM patients and 6 healthy subjects at the same time. Exosomes were isolated by qEVoriginal size exclusion column from plasma, and protein was extracted. Quantitative analysis to identify the difference in plasmaexosomal protein expression was performed by liquid chromatography-mass spectrometry(LC-MS), and the results were analyzedwith various bioinformatic tools to determine changes in activity features, subcellular localization and cellular functions of theidentified proteins. Results A total of 948 quantifiable protein molecules were obtained, 23 were up-regulated in the T2DMpatients as compared with the healthy subjects, and 124 were down-regulated. Among the up-regulated exosome components, manyproteins including several complement component molecules were inflammation-associated proteins. Conclusion There is acertain difference in the expression of plasma exosomal proteins between type 2 diabetic patients and normal healthy people. Thisdifference in exosomal protein composition may provide new candidate molecules for the early diagnosis of type 2 diabetes mellitusand provide evidence for the elucidation of its pathogenesis.
作者
张莉
黄彩艳
冯天元
杨书才
刘慧
张扬
王革非
ZHANG Li;HUANG Cai-yan;FENG Tian-yuan;YANG Shu-cai;LIU Hui;ZHANG Yang;WANG Ge-fei(Center of Pathogen Biology and Immunology,Shantou University Medical College,Shantou,Guangdong 515041,China;Pingshan District People’s Hospital/Pingshan General Hospital of Southern Medical University,Shenzhen,Guangdong 518118,China)
出处
《实用预防医学》
CAS
2022年第5期546-550,共5页
Practical Preventive Medicine
基金
国家重点研发计划资助(2020YFC2006400)
深圳市科技创新委员会基础研究项目,(JCYJ20160429173544879)。
