摘要
本实验以刺梨果渣为原料,采用酶法制备可溶性膳食纤维(soluble dietary fiber,SDF)、不可溶性膳食纤维(insoluble dietary fiber,IDF)和全膳食纤维(total dietary fiber,TDF),并采用动态高压微射流(dynamic high pressure microfluidization,DHPM)对刺梨果渣SDF进行改性处理,初步探索了刺梨果渣膳食纤维及DHPM处理SDF(DHPM-SDF)的理化特性及其对淀粉酶活力和葡萄糖扩散的影响。结果表明,IDF和SDF都能通过吸附葡萄糖、抑制葡萄糖扩散以及改变胰淀粉酶二级结构来减缓葡萄糖的流动进程和淀粉消化速率,其中,IDF对葡萄糖的吸附能力和抑制葡萄糖扩散能力分别是SDF的1.28倍和1.99倍,而SDF的胰淀粉酶活力抑制率是IDF的1.73倍,并且,SDF对胰淀粉酶活力的抑制作用主要通过改变胰淀粉酶的α-螺旋和无规卷曲结构。TDF表现出与IDF相似的葡萄糖吸附能力和抑制淀粉酶活力的能力。与SDF相比,DHPM-SDF平均粒径增加了2.08倍,使得其葡萄糖吸附能力和抑制葡萄糖扩散能力分别提高了28.13%和62.09%,并且DHPM-SDF能显著减少胰淀粉酶的α-螺旋和无规卷曲结构相对含量(P<0.05),其对淀粉酶活力的抑制能力是SDF的1.44倍。因此,刺梨果渣膳食纤维,尤其是SDF可作为降血糖产品开发的潜在优良资源,并且DHPM是提高刺梨果渣可溶性膳食纤维降血糖活性的有效改性处理手段。
Soluble dietary fiber(SDF),insoluble dietary fiber(IDF)and total dietary fiber(TDF)were prepared from Rosa roxburghii Tratt.pomace by enzymatic hydrolysis usingα-amlyase.Dynamic high pressure microfluidization(DHPM)was used to modify SDF,yielding DHPM-SDF.The physicochemical properties of IDF,SDF and DHPM-SDF,as well as their inhibitory effects on glucose diffusion andα-amylase activity were studied.It was shown that both IDF and SDF slowed down the glucose flow process by absorbing glucose and inhibiting its diffusion,and reduced starch digestibility by altering the secondary structure of pancreatic amylase.The ability of IDF to adsorb glucose and inhibit glucose diffusion was 1.28 and 1.99 times higher than that of SDF,respectively.However,the inhibitory effect of SDF against pancreatic amylase was 1.73 times higher than that of IDF,and SDF exerted its inhibitory effect mainly through changing theα-helix and random coil conformations of pancreatic amylase.The ability of TDF to adsorb glucose and inhibit amylase activity were similar to those of IDF.The average particle size of DHPM-SDF was 2.08-fold higher than that of SDF,so that the ability to adsorb glucose and inhibit glucose diffusion were increased by 28.13%and 62.09%,respectively.In addition,DHPM-SDF significantly reduced theα-helix and random coil conformations of pancreatic amylase(P<0.05),its amylase inhibitory activity is 1.44 times higher than that of SDF.In conclusion,dietary fibers from Rosa roxburghii Tratt.pomace,especially soluble dietary fiber,can be used as a good resource for the development of hypoglycemic products,and DHPM is an effective modification method to improve the hypoglycemic activity of SDF from Rosa roxburghii Tratt.pomace.
作者
官印珑
周丽妍
王辉
陈佳雨
明建
李富华
GUAN Yinlong;ZHOU Liyan;WANG Hui;CHEN Jiayu;MING Jian;LI Fuhua(College of Food Science,Southwest University,Chongqing 400715,China;Institute of Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第9期79-86,共8页
Food Science
基金
贵州省科技计划项目(黔科合支撑[2021]一般116)
西南大学“大学生创新创业训练计划”项目(X202010635434
X202010635437)。
关键词
刺梨果渣
膳食纤维
动态高压微射流
淀粉消化
葡萄糖扩散
Rosa roxburghii Tratt.pomace
dietary fiber
dynamic high pressure microfluidization
starch digestibility
glucose diffusion
