摘要
采用网络药理学方法分析红核妇洁洗液在抗感染过程中发挥功效的潜在靶点和通路,为红核妇洁洗液的机制研究提供参考。首先运用TCMSP、SwissTargetPrediction和CTD数据库检索得到红核洗液中92个化合物的潜在靶标,再检索DisGeNET数据库获得感染性疾病相关靶点,将两者整合去重,得到与抗感染相关的交集靶点合计143个。构建药材-成分-靶点网络后,采用Cytoscape研究该网络的拓扑结构,明确红核妇洁洗液抗感染的关键化合物和潜在作用靶标,主要涉及PARP1、PTGS1、ESR1、IDO1、NOS2、ALOX5、EGFR、PTGS2、HDAC6和JAK1等。将交集靶点导入David数据库进行分析,发现红核洗液主要通过脂多糖介导的通路、TNF通路、TLR通路和NF-kappa B通路等发挥抗感染的功效。此外,红核洗液中还能抑制病原菌入侵和繁殖、调节机体免疫应答、减轻炎症反应、缓解机体氧化应激损伤,从而发挥抗感染的作用。
The study aimed to explore the mechanism of anti-infection of Honghe Fujie Lotion(HHFJL)through network pharmacology,and provide a scientific reference for its clinical applica‐tion.92 compounds in HHFJL were identified by GC-MS in preliminary studies.The action targets were collected by TCMSP,Toxicogenomics Database(CTD)and SwissTargetPrediction,while the infection related targets were queried through DisGeNET database,and the“compound-disease”intersection targets were obtained using Venn Diagram.The herb-active ingredient-intersection target network was constructed and analysized by Cytoscape.A total of 1056 targets were collected for HHFJL,and 143 key targets were selected,such as PARP1,PTGS1,ESR1,IDO1,NOS2,ALOX5,EGFR,PTGS2,HDAC6,JAK1,and so on.The results from DAVID indicated that lipopolysaccharide mediated signaling pathway,NF-kappa B signaling pathway,Toll-like receptor signaling pathway and TNF signaling pathway were the key pathways,which were involved in anti-infective therapies.HHFJL may show therapeutic effects on infection probably through inhibiting pathogenic,regulating immune function,alleviating inflammation and oxidative damage based on multi-target and multi-pathway.
作者
饶鸿宇
李沛波
谌攀
史志恒
苏薇薇
RAO Hongyu;LI Peibo;CHEN Pan;SHI Zhiheng;SU Weiwei(Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-evaluation of Post-marketed TCM/Guangdong Key Laboratory of Plant Resources/School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)
出处
《中山大学学报（自然科学版）（中英文）》
CAS
CSCD
北大核心
2022年第3期21-27,共7页
Acta Scientiarum Naturalium Universitatis Sunyatseni
关键词
红核妇洁洗液
抗感染
网络药理学
Honghe Fujie Lotion
infection
network pharmacology
