摘要
研究林药猴耳环[Pithecellobium clypearia(Jack)Benth.]外植体的消毒方式及不同植物生长调节剂的不同浓度与不同组培方式对猴耳环组培苗生根及成活的影响,筛选出猴耳环外植体最适宜的消毒方式是先用75.00%乙醇处理1 min,再用5.00%的次氯酸钙溶液处理25 min,萌芽率能达70.00%;最适宜生根的植物生长调节剂配比为IBA 0.40 mg/L+NAA 0.10 mg/L,在该浓度下传统组培的生根率和移栽成活率分别达70.30%及92.20%,无糖组培的生根成活率和移栽成活率分别达100.00%及99.90%,最佳的种植基质体积配比为河沙+泥炭土(1∶1)和黄心土+河沙+泥炭土(1∶1∶1)。用无糖组培方式进行猴耳环苗期生根,比传统组培生根率更高,根系更发达,更适合进行商业化生产。
The effects of disinfection methods on explants of forest medicine Pithecellobium clypearia(Jack)Benth.,different concentrations of different plant growth regulators and different tissue culture methods on rooting and survival of tissue culture seedlings of Pithecellobium clypearia were studied. The most suitable disinfection method for explants of Pithecellobium clypearia was first treated with 75.00% ethanol for 1 min,and then treated with 5.00% calcium hypochlorite solution for 25 min. The germination rate was70.00%. The most suitable ratio of plant growth regulators for rooting was IBA 0.40 mg/L + NAA 0.10 mg/L. Under this concentration,the rooting rate and transplanting survival rate of traditional tissue culture were 70.30% and 92.20%,respectively. The rooting survival rate and transplanting survival rate of sugar-free tissue culture were 100.00% and 99.90%,respectively. The best planting substrate ratio was river sand + peat soil(1∶1)and loess + river sand + peat soil(1∶1∶1). The rooting rate of sugar-free tissue culture was higher than that of traditional tissue culture,and the root system was more developed,which was more suitable for commercial production.
作者
王立
陈祖旭
黄宏健
陈小玲
陈启民
黄世能
WANG Li;CHEN Zu-xu;HUANG Hong-jian;CHEN Xiao-ling;CHEN Qi-min;HUANG Shi-neng(Xinhui Forestry Science Institute,Jiangmen 529100,Guangdong,China;Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou 510520,China)
出处
《湖北农业科学》
2022年第9期151-155,共5页
Hubei Agricultural Sciences
基金
广东省林学会科技计划项目(2021-GDFS-KJ-02)
广州市林业和园林局项目(穗林园科信合[2016]03号)
广东省林业科技创新项目(2017KJCX004)。
