摘要
为实现当归种植中“化肥农药减量增效”的绿色发展目标,探讨化肥减量配施一种中药源植物生长调节剂对当归质量和根际土壤细菌群落的影响,为该中药源调节剂改善当归质量的机理提供科学依据。以当归为研究对象,设置常规量化肥(CK)、中药源调节剂配施化肥(T1)、中药源调节剂配施80%化肥(T2)、中药源调节剂配施60%化肥(T3)4个处理,测定当归5个生长期的生长指标、病情指标、产量、根际土壤理化指标等,并通过16S rDNA扩增子测序分析根际土壤细菌群落的变化。结果表明:(1)生长早期T1组和T2组的当归根长、芦头直径、地下生物量显著高于其他组,成药期CK组当归根部蚜虫出现率、蚜虫密度、病情等级、发病率均高于其他组,且产量最低,T2组产量最高,比CK组增产2.61倍;(2)全生长期当归根际土壤盐分、铵态氮含量、有效钾含量在组间差异不显著。有机质含量仅在苗期的CK组中高于其他组,其他时期组间无差异。有效磷含量在全生长期组间变化较大,pH值随着化肥减量逐渐增加;(3)全生长期当归根际土壤细菌群落多样性在组间无差异,门水平上群落结构组成一致,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、芽单胞菌门(Gemmatimonadetes)、酸杆菌门(Acidobacteria)为优势菌群;(4)属水平优势菌群为黄杆菌属(Flavobacterium)、鞘氨醇单胞菌属(Sphingomonas)、假单胞菌属(Pseudomonas)、拟杆菌属(Bacteroides)、MND1属、鞘氨醇杆菌属(Pedobacter)、柠檬酸杆菌属(Citrobacter)、Ellin6067属、芽单胞菌属(Gemmatimonas)、马赛菌属(Massilia)、鞘脂菌属(Sphingobium)、溶杆菌属(Lysobacter)、Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium属、Haliangium属和硝化螺旋菌属(Nitrospira)。其中假单胞菌属、柠檬酸杆菌属、Ellin6067属和马赛菌属丰度在当归不同生长时期存在组间显著差异,但仅有假单胞菌属丰度与当归根部生长指标显著正相关,与病情指标显著负相关,且CK组的假单胞菌属在早期和成药期较其他组显著降低。化肥减量配施中药源植物生长调节剂可促进当归早期根部生长,改善成药期根腐病病情,提高产量。该中药源调节剂通过增加当归根际土壤中假单胞菌属丰度促进当归质量的提升。
In order to achieve the green development goal of“reducing chemical fertilizer and increasing efficiency”in the cultivation of Angelica sinensis,the effects of chemical fertilizer reduction and application of plant growth regulators from traditional Chinese medicine on the quality and bacterial community in rhizosphere soil of A.sinensis were investigated,so as to provide scientific basis for the mechanism of improving the quality of A.sinensis.A.sinensis was taken as the research object.Four treatments were set up,including chemical fertilizer(CK),Chinese medicine source regulator combined with chemical fertilizer(T1),Chinese medicine source regulator combined with 80%chemical fertilizer(T2),and Chinese medicine source regulator combined with 60%chemical fertilizer(T3).The growth indexes,disease indexes,yield,rhizosphere soil physical and chemical indexes of A.sinensis in the 5 growth stages were determined,and the changes of bacterial community in the rhizosphere soil were analyzed by 16S rDNA amplification sequence.Results showed as below:(1)The root length,main root diameter and root biomass of A.sinensis in treatment T1 and T2 were significantly higher than those in other treatments at early growth stage.The aphid emergence rate,aphid density,disease grade and incidence rate of A.sinensis in CK were higher than those in other treatments,and the yield was the lowest in CK,the highest in T2,which was 2.61 times higher than that of CK.(2)There was no significant difference in the salinity,ammonium nitrogen and available potassium among the treatments in whole life growth period.The organic matter in CK at seedling stage was higher than that in other treatments,but there was no difference among treatments at other growth stages.The available phosphorus changed greatly in the whole growth period,and the pH value increased gradually with the reduction of chemical fertilizer.(3)There was no difference in the diversity of bacterial community in the rhizosphere soil of A.sinensis during the whole growth period.The community structure was consistent at phylum level.Proteobacteria,Bacteroidetes,Firmicutes,Actinobacteria,Gemmatimonadetes and Acidobacteria were the dominant phylum.(4)At the genus level,the dominant genera were Flavobacterium,Sphingomonas,Pseudomonas,Bacteroides,MND1,Pedobacter,Citrobacter,Ellin6067,Gemmatimonas,Massilia,Sphingobiu,Lysobacter,Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium,Haliangium,and Nitrospira.The abundance of Pseudomonas,Citrobacter,Ellin6067 and Massilia were significantly different among treatments in different growth stages of A.sinensis.However,only the abundance of Pseudomonas was positively correlated with the root growth index of A.sinensis,and negatively correlated with the disease index.The abundance of Pseudomonas in CK was significantly lower than that in other treatments in the early stage and drug preparation stage.Chemical fertilizer reduction and application of plant growth regulators from traditional Chinese medicine may promote the root growth of A.sinensis at early growth stages,improve the disease condition of root rot in the proprietary period,and increase the yield.The Chinese medicine source regulator may improve the quality of A.sinensis by increasing the abundance of Pseudomonas in the rhizosphere soil.
作者
谢田朋
柳娜
刘越敏
曲馨
薄双琴
景明
XIE Tian-peng;LIU Na;LIU Yue-min;QU Xin;BO Shuang-qin;JING Ming(School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000)
出处
《生物技术通报》
CAS
CSCD
北大核心
2022年第3期79-91,共13页
Biotechnology Bulletin
基金
国家自然科学基金项目(81660653)
甘肃省“三区”人才计划项目(219)
甘肃中医药大学引进人才科研启动基金项目(2018YJRC-03)
甘肃中医药大学科创基金重点项目(2019KCZD-3、2020KCZD-3)
甘肃省青年博士基金项目(2021QB-075)
甘肃省产业支撑计划项目(2021CYZC-13)。
关键词
当归
植物生长调节剂
16S
rDNA
细菌群落
根际土壤
Angelica sinensis
plant growth regulator
16S rDNA
bacterial community
rhizosphere soil
