摘要
目的:探讨莓茶决明子固体饮料对饮酒大鼠酒精肝损伤的保护作用及其机制。方法:43%vol白酒灌胃SD大鼠,建立酒精肝损伤大鼠模型,以低、中、高剂量的固体饮料灌胃模型大鼠,通过细胞毒性试验确定莓茶提取物和决明子提取物颗粒配比,通过试剂盒检测大鼠生理生化指标,再利用转录组学分析相关差异基因表达,评估莓茶决明子固体饮料对酒精肝损伤大鼠的影响并揭示作用机制。结果:与模型组相比,固体饮料组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性显著降低;白细胞介素6(IL-6),肿瘤坏死因子(TNF-α)水平极显著降低;肝脏还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)活性极显著升高,丙二醛(MDA)、甘油三酯(TG)含量显著降低,明显改善大鼠肝脏氧化应激状态和炎症反应。固体饮料组的肝脏病理切片与模型组相比,肝细胞炎症细胞浸润和空泡变性明显减少;转录组学分析结果表明spermidine synthase、Sirt 7、RT1-A2、RT1-CE1等基因的调节和氨基酸代谢等途径与莓茶决明子固体饮料缓解酒精肝损伤有关。结论:莓茶决明子固体饮料可通过改善肝功能、降低肝脏炎症反应和氧化应激水平缓解酒精诱导的肝损伤。
Objective:This study aimed to investigate the protective effect and mechanism of solid beverage with vine tea combined with and cassia on alcoholic liver injury in rats.Methods:SD rats were given 43%vol aging by gavage to establish model of alcoholic liver injury.The model was fed with low,medium and high doses of solid beverage.The appropriate ratio of solid beverages was detected by cell test,the physiological and biochemical indicators of rats were detected by using kits,and the expression of related differential genes was studied by transcriptomic analysis to assessing the effect of vine tea extract combined with cassia extract solid beverage on alcoholic liver injury in rats and revealing the mechanism of action.Results:Compared with the model group,the activities of ALT,AST and LDH in the solid beverage group were significantly decreased;the levels of IL-6 and TNF-αwas significantly reduced;the activities of GSH and SOD in the liver were significantly increased,while the contents of MDA and TG were significantly decreased;finally the oxidative stress and inflammatory response in rat liver were significantly improved.Compared to the model group,the inflammatory cell infiltration and vacuolar degeneration of liver cells in the liver pathological sections of the solid beverage group were significantly reduced;transcriptomic analysis speculated that the regulation of genes such as spermidine synthase,Sirt 7,RT1-A2,RT1-CE1 and other pathways and amino acid metabolism are related to vine tea extract combined with cassia extract solid beverage to alleviate alcoholic liver injury.Conclusion:Vine tea extract combined with cassia extract solid beverage can alleviate alcohol-induced liver injury by improving liver function,reducing liver inflammation and oxidative stress.
作者
湛莉
汤荃荃
张梓莹
石昱
滕建造
刘子龙
刘仲华
张盛
ZHAN Li;TANG Quan-quan;ZHANG Zi-ying;SHI Yu;TENG Jian-zao;LIU Zi-long;LIU Zhong-hua;ZHANG Sheng(College of Horticulture,Hunan Agricultural University,Changsha,Hunan 410128,China;National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients,Changsha,Hunan 410128,China;Hunan A-plus Biotech Co.,Ltd.,Changsha,Hunan 410301,China)
出处
《食品与机械》
北大核心
2022年第5期157-165,172,共10页
Food and Machinery
基金
湖南省重点研发计划项目(编号:2020NK2030,2020WK2017)。
