摘要
建立同步测定葡萄酒中的甜味剂阿斯巴甜、阿力甜和纽甜含量的高效液相色谱法。采用固相萃取法净化样品提取液,以乙腈和水作为流动相,色谱柱为Poroshell EC-C18(2.7μm,4.6mm×100mm)。结果显示,化合物在0.02 mg/L~3.00 mg/L范围内线性关系良好,相关系数大于0.9995。定量限均为0.1mg/kg。添加回收试验中,阿斯巴甜回收率为85.2%~93.5%,相对标准偏差(RSD)小于6%;阿力甜回收率为82.7%~91.5%,相对标准偏差(RSD)小于6%;纽甜回收率为90.3%~96.4%,相对标准偏差(RSD)小于4%。该方法快速、高效,准确,具有较大的应用潜力。
A method for the detection of aspartame,alitame and neotame in wine by HPLC was developed.The samples were purified by solid phase extraction.The mobile phase was acetonitrile and water solution.The separation column was Poroshell EC-C18(2.7μm,4.6mm×100mm).There were good linear correlations in the range of 0.02mg/L~3.00mg/L with the correlation coefficients more than 0.9995.The limits of quantification were 0.1mg/kg.The recoveries of aspartame were in the range of 85.2%~93.5%.The relative standard deviation of intraassay was less than 6%.The recoveries of alitame were in the range of 82.7%~91.5%.The relative standard deviation of intraassay was less than 6%.The recoveries of neotame were in the range of 90.3%~96.4%.The relative standard deviation of intraassay was less than 4%.The method was quick,efficient and accurate.
作者
季磊
陈博
马晓健
JI Lei;CHEN Bo;MA Xiao-jian(The Certification and Food and Drug Control Center of Dalian City,Dalian 116021,China)
出处
《宜春学院学报》
2022年第3期94-96,共3页
Journal of Yichun University
