摘要
为了解天津地区猪繁殖与呼吸综合征病毒(PRRSV)基因变异情况,2017年从天津某发病猪场采集的疑似PRRSV感染病料,接种Marc-145细胞分离到1株PRRSV毒株,将其命名为TJxq1701株;应用RT-PCR分段扩增出各基因片段,分别克隆到pMD-18T载体鉴定后测序。序列分析结果表明,TJxq1701株基因组全长15321 nt(不包括polyA尾),在分子遗传演化上与HP-PRRSV参考毒株JXA1和JXA1-P80的核酸同源性分别为99.2%和99.7%,与CH-la和VR2332的同源性为95.1%和89.5%,与NADC30同源性为84.4%,而与欧洲型Lelystad virus(LV)株的同源性仅为61.0%。Nsp2基因存在与HP-PRRSV参考毒株位置相同的90个碱基缺失;氨基酸位点分析表明在Nsp1-3、Nsp9-11、Gp2-Gp5存在至少10处以上与JXA1-P80相同的特征性位点突变,推测该分离毒株为JXA1-P80遗传演化毒株(类JXA1-P80毒株)。
In order to preliminarily understand the variation of porcine reproductive and respiratory syndrome virus(PRRSV)gene in Tianjin region,a strain of PRRSV,named TJxq1701 was isolated from a suspected pig and infected Marc-145 cells.The gene fragments of PRRSV TJxq1701 were amplified by RT-PCR,the fragments were sequenced and cloned into the pMD18-T vector,and the genomic length of the PRRSV TJxq1701 strain was 15321 bp(polyA tail removed),and the nucleotide homologies with HP-PRRSV strains JXA1 and JXA1-P80 were 99.2%and 99.7%,respectively.The homology with CH-la and VR2332 were 95.1%and 89.5%,the homologies with NADC30 was 84.4%,the homology with European Lelystad virus(LV)strain was only 61.0%.Nsp2 gene had 90 base deletions in the same position comparing with the HP-PRRSV reference strains.Amino acid site analysis showed that there were at least 10 characteristic site mutations in Nsp1-3,Nsp9-11 and Gp2-Gp5 between TJxq1701 and JXA1-P80,suggesting that the TJxq1701 strain was JXA1-P80 genetic evolution strain(JXA1-P80 like strain).
作者
杨婧
王欢欢
刘野
李文忠
石青青
付旭彬
李留安
孙英峰
YANG Jing;WANG Huan-huan;LIU Ye;LI Wen-zhong;SHI Qing-qing;FU Xu-bin;LI Liu-an;SUN Ying-feng(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China;Tianjin Agricultural KenKangjia Ecological Breeding Co.,Ltd,Tianjin,300380,China;Tianjin Ringpu Bio-Technology Co.,Ltd,Tianjin,300308,China)
出处
《动物医学进展》
北大核心
2022年第6期1-6,共6页
Progress In Veterinary Medicine
基金
天津市应用基础与前沿研究计划面上项目(19JCYBJC29800)。
关键词
猪繁殖与呼吸综合征病毒
遗传演化
类疫苗毒株
Porcine reproductive and respiratory syndrome virus
genetic evolution
vaccine strain
