摘要
蔗糖转运效率直接影响着水稻籽粒淀粉积累,进而影响水稻产量。目前已报道协调蔗糖转运速率的水稻蔗糖转运蛋白家族(OsSUTs)主要成员有5个基因,而且基因间存在相互促进或抑制的复杂关系,但对于它们间的互作作用机理尚不明晰。因此本研究根据水稻5个OsSUTs基因的序列信息,分别设计出靶标位点,并成功构建了相应的CRISPR/Cas9载体,通过转基因技术获得5个OsSUT基因的阳性敲除苗。研究结果为探究Os SUT基因的分子功能及其调节关系提供了良好材料。
The efficiency of sucrose transfe rdirectly affects starch accumulation in rice grains and rice yield.There are five sucrose transporters reported in rice,the relationships of them are complex,and the interaction mechanisms of them are still unclear.In this study,based on the sequence information of the five OsSUTs genes in rice,target sites were designed respectively,the corresponding CRISPR/Cas9 vector was successfully constructed,and five positive knock-out seedlings of OsSUTs genes were obtained through transgenic technology.It provides a good material for exploring the molecular function and regulatory relationship of OsSUTs gene.
作者
杨宏
陈进
张春龙
Khadija Tehseen Arshad
李秋平
王拓
文建成
李丹丹
Yang Hong;Chen Jin;Zhang Chunlong;Khadija Tehseen Arshad;Li Qiuping;Wang Tuo;Wen Jiancheng;Li Dandan(Rice Farming Research Institute,Yunnan Agricultural University,Kunming,650201;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,650201)
出处
《分子植物育种》
CAS
北大核心
2022年第7期2286-2293,共8页
Molecular Plant Breeding
基金
云南省院士工作站项目(2018IC065)
国家自然科学基金项目(32060137)共同资助。
