摘要
目的采用高效液相色谱法(HPLC)同时测定不同产地何首乌和不同炮制工艺制何首乌中大黄素8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚、游离蒽醌和结合蒽醌的含量。方法采用Agilent 5 TC-C_(18)(2)色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.1%甲酸水为流动相梯度洗脱,流速1.0 mL·min^(-1),检测波长254 nm,测定大黄素8-O-β-D-葡萄糖苷、大黄素和大黄素甲醚的含量;按照《中华人民共和国药典》(2020年版)中何首乌项下方法,测定游离蒽醌和结合蒽醌的含量。结果大黄素8-O-β-D-葡萄糖苷、大黄素和大黄素甲醚分别在0.6745~21.5845、0.4267~13.6550和0.2003~6.9685μg·mL^(-1)范围内具有良好线性关系(R^(2)≥0.9991),平均回收率分别为98.42%、100.08%和96.60%。不同产区何首乌大黄素8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚含量范围分别为0.13~2.62、0.08~0.13和0.05~0.72 mg·g^(-1);游离蒽醌和结合蒽醌含量范围分别为0.1~1.7和1.0~8.7 mg·g^(-1)。结论本方法操作简单、准确度高、重复性好,可以用于何首乌中蒽醌类成分含量测定。
Objective To simultaneously determine the contents of emodin-8-O-β-D glucoside,emodin,physcion,free anthraquinone and bound anthraquinone in Polygoni Multiflori Radix from different origins and processed with different methods.Methods The contents of emodin-8-O-β-D glucoside,emodin and physcion were determined on an Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm,5μm)column with a mobile phase composed of methanol and 0.1% formic acid solution with gradient elution at 1.0 mL·min^(-1).The detection wavelength was 254 nm,the column temperature 30℃,and the injection volume was 10μL.The contents of free anthraquinone and bound anthraquinone were determined using the method for Polygonum multiflorum specified in Chinese Pharmacopoeia(2020 Edition).Results Emodin-8-O-β-D glucoside,emodin,physcion had good linear relationships in the ranges of 0.6745~21.5845,0.4267~13.6550 and 0.2003-6.9685μg·mL^(-1),respectively(R^(2)≥0.9991).The recovery was 98.42%,100.08% and 96.60% respectively.The contents of emodin-8-O-β-D glucoside,emodin,physcion,free anthraquinone and bound anthraquinone in Polygoni Multiflori Radix from different origins were 0.13~2.62,0.08~0.13,0.05~0.72,0.1~1.7 and 1.0~8.7 mg·g^(-1),respectively.Conclusion This method is simple,accurate and reproducible,which can be used for the determination of anthraquinones in Polygonum multiflorum.
作者
高慧宇
杨建波
孙华
宋云飞
程显隆
王雪婷
魏锋
汪祺
王莹
靳洪涛
马双成
GAO Huiyu;YANG Jianbo;SUN Hua;SONG Yunfei;CHENG Xianlong;WANG Xueting;WEI Feng;WANGQi;WANG Ying;JIN Hongtao;MA Shuangcheng(National Institutes for Food and Drug Control,Beijing 100050,China;Institute of Materia Medica,Chinese Academy of Medical Science,Beijing 100050,China)
出处
《中国药物警戒》
2022年第5期522-526,共5页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金资助项目(81773874、81973476)。
关键词
何首乌
高效液相色谱法
蒽醌类成分
含量测定
产地
炮制工艺
Polygoni Multiflori Radix
high performance liquid chromatography
anthraquinones
content determination
Origin
processe
