摘要
目的利用3D打印技术制备生物活性玻璃/羟基磷灰石骨修复材料,观察复合支架的表面形貌特征,检测其力学性能、孔隙率及降解性能。方法分别采用生物活性玻璃(BG%)/羟基磷灰石(HA%)为BG0/HA100、BG10/HA90、BG20/HA80、BG30/HA70、BG40/HA60、BG50/HA50、BG100/HA0配比的混合液作为打印墨水,应用Wolfram Mathematica 9.0软件建模后利用3D打印技术制备复合支架。通过扫描电镜观察各组复合支架的外观结构、微观结构,计算支架孔隙率,检测抗压强度,最后进行体外降解实验。结果将不同配比的各组复合支架进行宏观测量孔径均为500μm;扫描电镜后观察得出配比为BG20/HA80组微观孔隙结构较为均匀;通过EDS测试复合支架表面钙磷比接近正常人体骨组织。通过阿基米德排水法测得,除BG100/HA0组外孔隙率均达到50%以上,符合人工骨修复材料的要求,BG20/HA80组与BG100/HA0组孔隙率差异有统计学意义(t=3.375,P=0.0279);力学分析结果得出,除BG0/HA100组、BG10/HA90组外抗压强度均在2 MPa以上,满足人体松质骨的应力要求;各组进行降解性实验得出,2周后BG20/HA80组的失重率是HA100/BG0组的2.7倍。结论利用3D打印技术制备BG20/HA80多孔生物活性玻璃/羟基磷灰石支架,孔隙率高,满足人体松质骨抗压性能要求,具有良好的降解性能,具有潜在临床应用价值。
Objective To prepare bioactive glass/hydroxyapatite bone repair materials by 3D printing technology,observe the surface morphology characteristics of composite scaffolds,and test their mechanical properties,porosity and degradation properties.Methods The ratios of bioactive glass(BG%)/hydroxyapatite(HA%)were BG0/HA100,BG10/HA90,BG20/HA80,BG30/HA70,BG40/HA60,BG50/HA50,BG100/HA0 respectively.The mixed solution was used as printing ink,and the composite scaffold was prepared by 3D printing technology after modeling with Wolfram Mathematica 9.0 software.The appearance structure and microstructure of each group of composite scaffolds were observed by scanning electron microscope,the porosity of the scaffolds was calculated,the compressive strength was detected,and finally the in vitro degradation experiment was carried out.Results The pore size of each group of composite scaffolds with different ratios was 500μm in macroscopic measurement;the microscopic pore structure of the BG20/HA80 group was observed by scanning electron microscope.The porosity measured by the Archimedes drainage method except the BG100/HA0 group reached more than 50%,which met the requirements of bone repair materials.The porosity difference between the BG20/HA80 group and the BG100/HA0 group was statistically significant(t=3.375,P=0.0279);the results of mechanical analysis showed that the compressive strength except the BG0/HA100 group and BG10/HA90 group were above 2 MPa,which met the stress requirements of human cancellous bone;the degradability experiments of each group showed that the BG20/HA80 group was in 2 MPa.After 2 weeks,the weight loss rate of BG20/HA80 group was 2.7 times that of BG0/HA100 group.Conclusion BG20/HA80 bioactive glass/hydroxyapatite scaffolds can be prepared by 3D printing technology.The scaffolds have high porosity,meet the requirements of human cancellous bone compressive properties,and have good degradation properties.It is a kind of potential clinical application value.
作者
王鑫
赵帆
甄平
WANG Xin;ZHAO Fan;ZHEN Ping(Ningxia Medical University,Yinchuan 750004,China;Gansu Provincial People's Hospital,Lanzhou 730000,China;Northwest University for Nationalities,Lanzhou 730030,China;The 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Lanzhou 730000,China)
出处
《宁夏医科大学学报》
2022年第5期511-516,共6页
Journal of Ningxia Medical University
基金
中央高校基本科研项目(31920200017)
甘肃省科技计划项目(20JR5RA588)。
