摘要
The effect of different combinations of botanical spices such as clove and nutmeg in different proportion on the antioxidants activities which include lipid peroxidation(LPO),superoxide dismutase(SOD),catalase(CAT),glutathione-S-transferase(GST)and glutathione peroxide(GPX)in juveniles and adults sizes of Clarias gariepinus was investigated using different combinations of clove(C),and nutmeg(C.N 0:0-Control 0%of Clove and Nutmeg;C.N 1:3-25%Clove and 75%Nutmeg;C.N 3:1-75%Clove and 25%Nutmeg;C.N 2:2-50%Clove and 50%Nutmeg;C.4-100%Clove;N4-100%)in triplicates.The results from the study indicated that the anaesthetic caused a substantial(p<0.05)modifications in the five antioxidants under examination.The highest deviations in the studied antioxidants were observed in the fish exposed to C4 combination of the anaesthetics and the lowest in the control.The results from this work therefore suggest that the anaesthetics can alter antioxidants levels in the fish which was more noticeable in the fish exposed to C.N 3:1-75%Clove and 25%Nutmeg;C.N 2:2-50%Clove and 50%Nutmeg;C.4-100%Clove;N4-100%.Hence fish farmers and scientists are advised to take caution when combining these plant extracts for use in aquaculture.
