摘要
目的 通过检测胆石症患者血清中的胆汁酸谱,探讨胆石症患者血清胆汁酸谱的检测在胆石症临床诊断及早期预防中的应用价值。方法 收集2019年9月至2020年6月在河北医科大学第一医院(以下简称“我院”)住院的48例胆囊结石患者(胆囊结石组)、11例胆管结石患者(胆管结石组)及26例同期在我院健康体检者(对照组)的血清,利用高效液相色谱串联质谱法测定其胆汁酸中的15种亚组分[胆酸(CA)、鹅脱氧胆酸(CDCA)、脱氧胆酸(DCA)、石胆酸(LCA)、甘氨胆酸(GCA)、甘氨熊脱氧胆酸(GUDCA)、甘氨鹅脱氧胆酸(GCDCA)、甘氨脱氧胆酸(GDCA)、牛磺胆酸(TCA)、牛磺脱氧胆酸(TDCA)、牛磺熊脱氧胆酸(TUDCA)、牛磺鹅脱氧胆酸(TCDCA)、牛磺石胆酸(TLCA)、甘氨石胆酸(GLCA)、熊脱氧胆酸(UDCA)],并进行比较。结果 三组GCA、GUDCA、GCDCA、TCA、TUDCA、TCDCA这6种胆汁酸亚组分比较,差异有统计学意义(P <0.05);胆囊结石组和胆管结石组中的GCA、GUDCA、TCA、TCDCA及TUDCA显著高于对照组,差异有高度统计学意义(P <0.01),但胆囊结石组与胆管结石组GCA、GUDCA、TCA、TCDCA及TUDCA比较,差异无统计学意义(P> 0.05);胆囊结石组GCDCA高于对照组,差异有统计学意义(P <0.05),但胆管结石组与对照组、胆囊结石组与胆管结石组之间GCDCA比较,差异无统计学意义(P> 0.05)。结论 血清胆汁酸代谢谱的检测,可以作为胆石症临床诊断及早期预防的重要标志物。
Objective To investigate the value of serum bile acid spectrum in clinical diagnosis and early prevention of cholelithiasis by detecting the serum bile acid spectrum in patients with cholelithiasis. Methods Serum of 48 patients with gallstone(gallstone group), and 11 patients with bile duct stone(bile duct stone group) hospitalized in the First Hospital of Hebei Medical University(hereinafter referred to as “our hospital”) from September 2019 to June 2020 and26 healthy subjects(control group) in our hospital during the same period were collected. The 15 subcomponents of bile acids(choleic acid [CA], chenodesoxycholic acid [CDCA], deoxycholic acid [DCA], lithocholic acid [LCA], glycocholic acid [GCA], glycoursodeoxycholic acid [GUDCA], glycochenodeoxycholic acid [GCDCA], glycodeoxycholic acid [GDCA],taurocholic acid [TCA], taurodeoxycholic acid [TDCA], tauroursodeoxycholic acid [TUDCA], taurochenodeoxycholic acid[TCDCA], taurolithocholic acid [TLCA], glycocholic acid [GLCA], ursodeoxycholic acid [UDCA]) were determined by high performance liquid chromatography tandem mass spectrometry and compared. Results There were statistically significant differences in six bile acid subcomponents(GCA, GUDCA, GCDCA, TCA, TUDCA, and TCDCA) among the three groups(P < 0.05). GCA, GUDCA, TCA, TCDCA, and TUDCA in gallstone group and bile duct stone group were significantly higher than those in control group, and the differences were highly statistically significant(P < 0.01). However, there were no significant differences in GCA, GUDCA, TCA, TCDCA, and TUDCA between the gallstone group and the bile duct stone group(P > 0.05). The GCDCA of gallstone group was higher than that of control group, the difference was statistically significant(P < 0.05). However, there was no significant difference in GCDCA between the bile duct stone group and the control group or between the gallstone group and bile duct stone group(P > 0.05). Conclusion The detection of serum bile acid metabolism spectrum can be an important marker for clinical diagnosis and early prevention of cholelithiasis.
作者
金素丽
武艳
汪冉
杨利
闫国超
赵帅
JIN Suli;WU Yan;WANG Ran;YANG Li;YAN Guochao;ZHAO Shuai(Inspection Center,the First Hospital of Hebei Medical University,Hebei Province,Shijiazhuang050000,China;Clinical Laboratory,the Second Hospital of Hebei Medical University,Hebei Province,Shijiazhuang050000,China)
出处
《中国医药导报》
CAS
2022年第13期165-168,172,共5页
China Medical Herald
基金
河北省卫生健康委员会医学科学研究课题(20190453)。
关键词
胆石症
胆囊结石
胆汁酸谱
高效液相色谱串联质谱
Cholelithiasis
Gallstone
Bile acid spectrum
High performance liquid chromatography tandem mass spectrometry
