摘要
目的探讨长链非编码RNA(lncRNA)淋巴细胞白血病缺失基因2(DLEU2)在舌鳞癌中的表达及其对癌细胞增殖和侵袭的影响。方法2018年5月至2019年7月收集在邯郸市第三医院进行手术治疗并经病理确诊的22例舌鳞癌组织及对应的癌旁组织标本。采用实时荧光定量PCR检测DLEU2在舌鳞癌组织和细胞中的表达情况。将体外培养的舌鳞癌细胞株CAL27细胞分为对照组(未转染)、空载体组(空载体)和DLEU2组(转染DLEU2),采用实时荧光定量PCR检测细胞中DLEU2表达,MTT法检测细胞增殖,Transwell检测细胞侵袭。结果与癌旁组织(1.00±0.00)相比,舌鳞癌组织中DLEU2表达水平(0.30±0.02)明显降低(P<0.05);3种舌鳞癌SCC-15(0.35±0.04)、SCC-4(0.48±0.04)和CAL-27(0.26±0.03)细胞中DLEU2的表达水平较人正常口腔黏膜HOK细胞(1.00±0.00)均明显降低(P<0.05)。与对照组相比,DLEU2组细胞中DLEU2表达水平明显升高,且细胞增殖和侵袭能力明显减弱(P<0.05)。结论lncRNA DLEU2在舌鳞癌组织和细胞中低表达,上调其表达可抑制舌鳞癌CAL-27细胞增殖和侵袭。
Objective To investigate the expression of long-chain non-coding RNA(lncRNA)deleted in lymphocytic leukemia 2(DLEU2)in tongue squamous cell carcinoma and its effect on the proliferation and invasion of cancer cells.Methods Cancerous tissue and corresponding paracancerous tissue specimens were collected from 22 confirmed cases of tongue squamous cell carcinoma who underwent surgery in The Third Hospital of Handan City from May 2018 to July 2019.The expression of DLEU2 in tongue squamous cell carcinoma cells and tissues were detected by real-time fluorescence quantitative PCR.The tongue squamous cell carcinoma cell line CAL-27 cells cultured in vitro were assigned into control group(untransfected),empty vector group(empty vector)and DLEU2 group(transfected with DLEU2).The expression level of DLEU2 was detected by real-time fluorescent quantitative PCR,cell proliferation by MTT method,and cell invasion by the Transwell assay.Results Compared with adjacent tissues,the expression level of DLEU2 in cancerous tissues was significantly reduced[(0.30±0.02)vs.(1.00±0.00),P<0.05].The expression levels of DLEU2 in cells of SCC-15,SCC-4 and CAL-27 were significantly reduced in comparison with DLEU2 level in normal human oral keratinocytes(HOK)[(0.35±0.04),(0.48±0.04),(0.26±0.03)vs.(1.00±0.00);P<0.05].Compared with the control group,the expression level of DLEU2 in the cells of the DLEU2 group was significantly increased,and the cell proliferation and invasion capacities were significantly reduced(P<0.05).Conclusions The expression of lncRNA DLEU2 was low expressed in cells and tissues of tongue squamous cell carcinoma.Upregulation of its expression can inhibit the proliferation and invasion of tongue squamous cell carcinoma CAL-27 cells.
作者
冯维
尚张丽
吴丽玮
FENG Wei;SHANG Zhangli;WU Liwei(Department of Stomatology,The Third Hospital of Handan,Handan,Hebei 056000,China;Department of Orthodontics,Handan Stomatological Hospital,Handan,Hebei 056001,China;Department of Stomatology,Shijiazhuang Third Hospital,Shijiazhuang,Hebei 050011,China)
出处
《安徽医药》
CAS
2022年第6期1231-1234,I0005,共5页
Anhui Medical and Pharmaceutical Journal
