摘要
比较了乳糖等3种双糖或核糖等3种单糖及其复配物对近中性范围共轭亚油酸(CLA)形成脂肪酸囊泡(FAV)的影响.用激光丁达尔效应确定FAV的pH窗口及各相区,用透射电子显微镜及动态光散射表征其形貌和尺寸,用浊度法研究了其稳定性,用等温滴定量热证明各种糖及其复配物与FAV表面的弱非共价键合作用,并经理论计算获得结合能.实验和计算结果表明,各种糖及其复配物均可以双向拓宽CLA形成FAV的pH窗口,且拓宽其近中性pH窗口的能力按照双糖<单糖≈双糖/单糖≤单糖/单糖的顺序依次增强.主要归结为单糖在FAV表面的强竞争吸附,以及双糖可能因多结合位点吸附而减少其自由羟基与环境水分子的缔合作用,从而影响多羟基小分子依靠自由羟基增强囊泡表面亲水性的效果.
Effects of three disaccharides such as lactose and three monosaccharides such as ribose and their mix⁃tures on the formation of fatty acid vesicles(FAV)of conjugated linoleic acid(CLA)in the near-neutral pH range were compared.The pH windows of FAV and phase regions were determined by laser Tyndall effect,the morphology and size of FAV were characterized by transmission electron microscopy and dynamic light scattering,the stabilities of FAV were studied by turbidimetry,the weak non-covalent binding of various saccharides and their mixtures on the FAV surface was proved by isothermal titration calorimetry,and the binding energies were calculated theoretically.The experimental and computational results show that all saccharides and their mixtures can both-side extend the pH window of FAV,and the near neutral-side pH window extends in the following order:disaccharide<monosaccha⁃ride≈disaccharide/monosaccharide≤monosaccharide/monosaccharide.It mainly comes down to the stronger competitive adsorption of monosaccharides than disaccharides on the FAV surface.Additionally,the possible multi-binding site adsorption of disaccharides on FAV surface may result in weaker association between the free hydroxyl groups of disaccharides and environmental water molecules than monosaccharides,which makes negative contribution to the enhanced hydrophilicity of the FAV surface relying on polyhydroxyl groups of small molecules.
作者
李磊
方云
夏咏梅
薄纯玲
樊晔
LI Lei;FANG Yun*;XIA Yongmei;BO Chunling;FAN Ye(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Synthetic and Biological Colloids(Ministry of Education),School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2022年第4期66-73,共8页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21606107)
国家重点研发计划项目(批准号:2017YFB308705)资助。
关键词
脂肪酸囊泡
共轭亚油酸
双糖
单糖
pH窗口
Fatty acid vesicle
Conjugated linoleic acid
Disaccharide
Monosaccharide
pH Window
