摘要
从水稻根中分离出的内生细菌HMC50通过16S rRNA基因和全基因组序列分析鉴定为久留里副伯克霍尔德菌(Paraburkholderia kururiensiss)。在基因组水平预测到该菌株具有与促进植物生长、鞭毛介导的运动性和解毒作用相关的主要特征,发现与固氮相关的18个基因中包括关键基因nifH以及色氨酸合酶α链(EC 4.2.1.20)基因和色氨酸合酶β链(EC 4.2.1.20)基因,因此推测该菌株具有固定N_(2)和分泌生长素(IAA)的能力;通过植物促生特性试验的验证,发现HMC50的确具有分泌IAA的能力(29.57 mg/L)并且具有较高的固氮酶活性11.9nmol/(mL·h),除此之外,该菌株同时具有产铁载体的能力,定量检测A/Ar是0.29,1-氨基环丙烷羧酸脱氨酶活性为0.35 U/mg,溶解无机磷的能力较强(菌落溶磷透明圈直径HD与菌落直径CD的比值为3.97);盆栽试验发现该菌株对水稻有促进生长的潜能,接菌后水稻幼苗的根长、茎长、根重以及总鲜重上都优于空白对照组(P<0.05)。以上研究结果表明,Paraburkholderia kururiensis HMC50具有优良的功能特性,可以显著促进水稻幼苗生长,该研究结果可为水稻特用的微生物肥料研制提供优良的菌株资源。
The endophytic bacterial HMC50 was isolated from rice roots and identified as paraburkholderia kururiensis by 16S rRNA gene and whole genomic analysis.The strain was predicted to have the main features associated with the promotion of plant growth,flagella-mediated motility and detoxification at the genomic level.In the prediction results,18 genes related to nitrogen fixation were found to include the key gene nifH,as well as tryptophan synthase alpha chain(EC 4.2.1.20)and tryptophan synthase beta chain(EC 4.2.1.20)genes,so it is speculated that this strain could fix N_(2) and secrete IAA.The results of experiment on plant growth-promoting properties verified that HMC50 had the ability to secrete IAA(29.57mg/L)and had a high nitrogenase activity of 11.9 nmol/(mL·h).In addition,this strain also had the ability to produce siderophores,and the quantitative detection A/Ar was 0.29,the activity of ACC deaminase was 0.35 U/mg,and the ability of dissolve inorganic phosphorus is strong,and the ratio of the diameter of the transparent circle phosphorus-soluble of colony to the diameter of the colony is 3.97.The pot experiment showed that the strain had the potential to promote the growth of rice,and it was better than the blank control group in terms of root length,stem length,root weight and total fresh weight of seedlings(P<0.05).The above results show that strain Paraburkholderia kururiensis HMC50 has excellent plant beneficial characteristics.The results are helpful for the development of microbial fertilizer for rice.
作者
王星
孟炯放
马荣
何山文
郭鹤宝
张晓霞
WANG Xing;MENG Jiong-fang;MA Rong;HE Shan-wen;GUO He-bao;ZHANG Xiao-xia(Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;Shanghai Academy of Landscape Architecture Science and Planning,Shanghai 200232)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2022年第4期218-228,共11页
Soil and Fertilizer Sciences in China
基金
国家自然科学基金项目(31970007)
中国农业科学院科技创新工程(CAAS-ZDRW202201)。
关键词
久留里副伯克霍尔德菌
促生功能
内生细菌
全基因组序列分析
Paraburkholderia kururiensis
plant growth promotion
bacterial endophyte
whole genome sequence analysis
