摘要
黄花菜是我国传统特色蔬菜干制品。为了挖掘黄花菜功能成分,采用硝酸调节水pH至1.8,提取黄花菜多糖,用Sevag法脱除蛋白,对黄花菜多糖基本特性、理化性质和体外抗氧化活性进行了分析。结果表明:脱除蛋白后黄花菜多糖得率为0.72%,多糖含量为34.91%,半乳糖醛酸含量为26.42%。黄花菜多糖含有高酯果胶,甲酯化程度值为96.13%±0.3%。黄花菜多糖中性糖分主要由半乳糖、葡萄糖、木糖组成,其摩尔比为62.50:17.31:11.30。显微观察发现,黄花菜多糖有絮状结构,表面不平整、不连续。傅里叶变换红外光谱(Fourier transform infrared,FTIR)表明,黄花菜多糖中含有较多的酯键。流变学特性分析表明,随着剪切速率增加,黄花菜多糖溶液的表观黏度先快速下降随后维持在较低水平;在各浓度下,其G’均大于G’’。黄花菜多糖峰1分子量较高,Mw为1310.32 kDa;峰2分子量相对小很多,Mw为83.81 kDa。抗氧化分析表明黄花菜多糖具有较强的2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基(2,2’-Azinobi-(3-ethylbenzthiazoline-6-sulphonate) radical,ABTS;·)清除能力和铁离子还原能力(Ferric reducing antioxidant power,FRAP)。研究结果为深入了解黄花菜多糖理化性质及其生物活性的开发利用提供了依据。
Daylily is a traditional and typical dry product in our country. In order to explore the functional components from daylily, daylily polysaccharides were extracted with water adjusted to pH1.8 using nitric acid. Proteins were removed by the Sevag method. The basic characteristics, physical and chemical properties and in vitro antioxidant activity of the daylily polysaccharides were analyzed. The results showed that the yield of daylily polysaccharides after protein removal was 0.72%, the polysaccharide content was 34.91%, and the galacturonic acid content was 26.42%. Daylily polysaccharide contains high-ester pectin, and the degree of methyl esterification was 96.13%±0.3%. The neutral sugar of daylily polysaccharide was mainly composed of galactose, glucose and xylose, and its molar ratio was 62.50:17.31:11.30.Microscopic observation revealed that the daylily polysaccharide had a flocculent structure, and the surface was uneven and discontinuous. The Fourier transform infrared(FTIR) diagram showed that the daylily polysaccharides contained more ester bonds. Analysis of the rheological properties showed that as the shear rate increased, the apparent viscosity of the daylily polysaccharide solution decreased rapidly and then maintained at a low level. At each concentration, its G’ was greater than G’’. The molecular weight of daylily polysaccharide peak 1 was relatively high, with Mw of 1310.32 kDa. Peak 2 molecular weight was relatively small, with Mw of 83.81 kDa. The rheological properties of the daylily polysaccharide solution showed that the apparent viscosity of the polysaccharide decreased rapidly as the shear rate increased, its G’ was greater than G" at all concentrations. Results from antioxidant analysis showed that daylily polysaccharides possessed strong 2,2’-azinobi-(3-ethylbenzthiazoline-6-sulphonate) radical(ABTS;·) scavenging ability and Ferric reducing antioxidant power(FRAP) ability. This study could provide a profound understanding on the physical and chemical properties of daylily polysaccharides and provide a basis for the development and utilization of their biological activities.
作者
刘艺珠
刘佩冶
赵玉梅
曹建康
LIU Yizhu;LIU Peiye;ZHAO Yumei;CAO Jiankang(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;College of Chemistry and Materials Engineering,Beijing Industrial and Commercial University,Beijing 100048,China)
出处
《食品工业科技》
CAS
北大核心
2022年第12期54-61,共8页
Science and Technology of Food Industry
基金
中国农业大学省校合作项目(201904710611628)
中国农业大学2115人才工程(1061-00109019)
中国农业大学北京学院项目。
关键词
黄花菜多糖
得率
理化性质
抗氧化活性
daylily polysaccharide
yield
physical and chemical properties
antioxidant activity
