摘要
为了提高苏云金芽胞杆菌的芽胞产量,应用响应面设计对苏云金芽胞杆菌(Bacillus thuringiensis,Bt)JQD117进行发酵培养基和摇瓶发酵条件的优化。本研究在单因素试验基础上,采用响应面试验设计方法优化培养基组分和发酵条件参数。最佳发酵培养基为棉籽饼粉2.00%,大豆饼粉1.00%,酵母粉1.50%,可溶性淀粉2.00%,CaCO30.30%,MnSO40.04%,MgSO_(4)0.18%,K2HPO40.04%;最佳发酵条件:接种量3%、装液量60 mL、温度30℃、转速150 r/min、初始pH 7.5。在最佳发酵条件下理论活芽胞产量可以达到6.75×10^(9)个/mL,试验验证后实际活芽胞产量为5.97×10^(9)个/mL。运用响应面法对Bt的发酵条件进行优化,获得了提高芽胞产量的关键参数,为该菌株产业化、规模化应用奠定了基础,为韭菜迟眼蕈蚊的绿色防控和蔬菜的安全生产提供了理论基础。
Aiming to increase living spore production of a Bacillus thuringiensis(Bt)strain JQD117,we employed response surface analysis methods to optimize the culture medium and the flask fermentation conditions.Based on preliminary results from single factor experiments,we used response surface analysis to model and optimize the components of culture medium and parameters of flask fermentation conditions.The best ratios of each single factors for the strain Bt JQD117 were 2.00%cotton seed cake meal,1.00%soybean meal,1.50%yeast extract,2.00%soluble starch,0.30%CaCO3,0.04%MnSO4,0.18%MgSO_(4),and 0.04%K2HPO4.For fermentation conditions,the optimum parameters were 3%inoculation amount,60 mL liquid volume in flask,150 r/min at 30°C,and pH 7.5.Theoretically,the living spore can reach 6.75×10^(9) spore/mL under this optimized condition.The verification experiment showed that the living spore yield was 5.97×10^(9) spore/mL.Our results have placed a critical foundation and theoretical basis for large-scale application of strain JQD117 for green control of Bradysia odoriphaga and the safe production of vegetables such as leeks.
作者
宋健
张海剑
丰硕
程佳旭
柳健虎
郭玮冰
曹伟平
SONG Jian;ZHANG Haijian;FENG Shuo;CHENG Jiaxu;LIU Jianhu;GUO Weibing;CAO Weiping(Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs,Baoding 071000,China)
出处
《中国生物防治学报》
CSCD
北大核心
2022年第2期333-341,共9页
Chinese Journal of Biological Control
基金
河北省重点研发项目(19226510D,21326505D)
河北省农村科学院现代农业科技创新工程(2019-1-03)
河北省农林科学院科技创新专项课题(2022KJCXZX-ZBS-6)。
关键词
韭菜迟眼蕈蚊
苏云金芽胞杆菌
发酵
单因素试验
响应面法
Bradysia odoriphaga
Bacillus thuringiensis
fermentation
single-factor experiment
response surface analysis
