摘要
针对黄瓜细菌性软腐病发生严重且难以防治的问题,本研究以活体防治效果为指标,筛选更加有利于田间应用的高效生防菌株。采用梯度稀释法分离纯化病害发生严重地块的健康黄瓜植株根际中的细菌菌株,再利用双层培养法筛选到6株黄瓜细菌性软腐病菌拮抗菌株,最终通过喷雾接种法筛选得到能够有效防治黄瓜细菌性软腐病的生防菌株ZF390,其防效可达77.94%,且该菌株能够有效抑制8种病原细菌的生长,抑菌谱广。结合形态学特征、生理生化特性和多位点序列分型分析,鉴定菌株ZF390为皮尔瑞俄类芽胞杆菌Paenibacillus peoriae。采用单因素试验对菌株ZF390摇瓶发酵工艺进行优化,最终发酵液菌体浓度提高了4个数量级,可达2.94×10^(9) CFU/mL。菌株ZF390是一株能够有效防治黄瓜细菌性软腐病的生防菌株,具有进一步开发成生防制剂的应用潜力。
In recent years,cucumber bacterial soft rot caused by Pectobacterium brasiliense(Pbr)occurred severely and was difficult to control.In this study,the control effect in greenhouse was used to screen the biocontrol agent strain suitable for field applications.The diluted plating method was used to separate and purify rhizosphere bacteria of healthy cucumber plants collected from severely diseased plots.Six antagonistic strains of Pbr were screened by the double-layer culture method.The result of spray infection method indicated that the disease incidence of cucumber inoculated with strain ZF390 was significant reduced and the control efficacy was 77.94%.Meanwhile,ZF390 exhibited significant and broad inhibition spectrum against eight pathogenic bacteria.The strain ZF390 was identified as Paenibacillus peoriae based on morphological traits,physiological and biochemical characteristics and multilocus sequence analysis.The fermentation process of strain ZF390 was optimized by single factor test,and the concentration of the fermentation broth was 104 times up to 2.94×10^(9) CFU/mL.In summary,the strain ZF390 is an effective biocontrol strain against cucumber bacterial soft rot and has the potential for further development into biocontrol formulations.
作者
赵昱榕
谢学文
许帅
谢华
石延霞
柴阿丽
李磊
李宝聚
ZHAO Yurong;XIE Xuewen;XU Shuai;XIE Hua;SHI Yanxia;Chai Ali;LI Lei;LI Baoju(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Beijing Agro-Biotechnology Research Center/Beijing Academy of Agriculture and Forestry Sciences,Beijing 100089,China)
出处
《中国生物防治学报》
CSCD
北大核心
2022年第2期476-486,共11页
Chinese Journal of Biological Control
基金
国家自然科学基金(31701753)
中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS)
农业部园艺作物生物学与种质创制重点实验室项目。
