摘要
目的:运用回顾性数据挖掘之关联规则分析观察健脾清热通络法对类风湿关节炎(RA)湿热痹阻证患者circRNA0003353表达及免疫炎症、骨代谢指标的影响。方法:纳入我院风湿科住院RA湿热痹阻证患者30例及正常对照组25例,收集外周血单个核细胞(PBMCs)及血清,检测circRNA0003353的表达、骨代谢指标(BALP、OC、OPG、RANKL)和免疫炎症指标(ESR,CRP,RF,IgA,IgG,IgM,C3,C4),运用回顾性数据挖掘之关联规则分析健脾清热通络法,即新风胶囊(XFC)联合黄芩清热除痹胶囊(HQC)对其的影响。结果:①与正常对照组相比,RA湿热痹阻证患者circRNA0003353、RANKL、ESR、CRP、RF、CCP-AB、IgA、IgG、IgM、C3和C4的表达显著升高(P<0.05),OC、BALP、OPG的表达显著降低(P<0.05);②Spearman相关性分析表明,RA湿热痹阻证患者circRNA0003353与ESR、RF、RANKL呈正相关(P<0.05);③与健脾清热通络中药、XFC联合HQC治疗前相比,RA湿热痹阻证患者circRNA0003353的表达降低(P<0.05),骨代谢指标RANKL降低,OC、OPG、BALP降低(P<0.05),免疫炎症指标ESR、CRP、CCP-AB、RF、IgG降低(P<0.05);④关联规则结果显示,XFC+HQC与circRNA0003353表达下降,骨代谢指标RANKL下降,BALP、OC、OPG上升,免疫炎症指标ESR、CRP、RF、IgG指标下降的支持度均>35%、置信度均>85%、提升度均>1,具有强关联关系。结论:健脾清热通络法(XFC联合HQC)治疗可降低RA湿热痹阻证患者circRNA0003353表达和免疫炎症指标,调节骨代谢指标。
Objective:To analyze the effect of Jianpi Qingre Tongluo method on the expression of circRNA0003353,immune inflammation and bone metabolism in patients with rheumatoid arthritis(RA)with damp-heat arthralgia syndrome using retrospective data mining association rules analysis.METHODS:Thirty patients with damp-heat arthralgia syndrome of RA and 25 normal control groups were included in the rheumatology department of our hospital.RANKL)and immune inflammatory indicators(ESR,CRP,RF,IgA,IgG,IgM,C3,C4),using retrospective data mining association rules to analyze Jianpi Qingre Tongluo method,that is,Xinfeng Capsule(XFC)combined with Scutellaria baicalensis to clear heat The effect of Bi Capsule(HQC)on it.Results:①Compared with the normal control group,the expressions of circRNA0003353,RANKL,ESR,CRP,RF,CCP-AB,IgA,IgG,IgM,C3 and C4 in RA patients with damp-heat arthralgia syndrome were significantly increased(P<0.05).The expressions of OC,BALP,and OPG were significantly decreased(P<0.05);②Spearman correlation analysis showed that circRNA0003353 was positively correlated with ESR,RF,and RANKL in RA patients with damp-heat obstruction syndrome(P<0.05);③was associated with Jianpi Qingre Tongluo Compared with before treatment with traditional Chinese medicine,XFC combined with HQC,the expression of circRNA0003353 in RA patients with damp-heat obstruction syndrome was decreased(P<0.05),the bone metabolism index RANKL,OC,OPG,BALP were decreased(P<0.05),and the immune inflammatory indexes ESR and CRP were decreased.,CCP-AB,RF,IgG decreased(P<0.05);④The results of association rules showed that the expression of XFC+HQC and circRNA0003353 decreased,the bone metabolism index RANKL decreased,BALP,OC,OPG increased,and the immune inflammatory indexes ESR,CRP,RF,IgG index decreased support>35%,confidence>85%,improvement>1,with strong correlation.Conclusion:The method of strengthening spleen and clearing heat and dredging collaterals(XFC combined with HQC)can reduce the expression of circRNA0003353 and immune inflammatory indexes,and regulate bone metabolism indexes in RA patients with damp-heat arthralgia syndrome.
作者
王杰
刘健
文建庭
王馨
WANG Jie;LIU Jian;WEN Jianting;WANG Xin(The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China)
出处
《中医药临床杂志》
2022年第4期725-730,共6页
Clinical Journal of Traditional Chinese Medicine
基金
科技部国家重点研发计划中医药现代化研究重点专项(2018YFC1705204)
国家自然基金面上项目(81973655)
安徽省高校协同创新项目(GXXT-2020-025)
安徽省重点研究和开发计划对外科技合作项目(201904b11020011)
安徽省省级质量工程教学研究项目(2018jyxm1068)
安徽省名中医刘健工作室建设项目(中医药发展秘[2018]11号)
全国中医药创新骨干人才培训项目(国中医药人教函[2019]128号)
安徽省重点研究与开发计划项目(201904a07020004)
安徽现代中医内科应用基础与开发研究省级实验室(2016080503B041)
安徽省第12批“115”创新团队(皖人才办[2019]1号)。
