摘要
水稻细菌性条斑病(BLS)是水稻主要病害之一。挖掘水稻细菌性条斑病抗性基因/数量性状位点(QTL),为培育水稻抗病品种提供参考依据。以多生育期表现高抗BLS且抗病性稳定的栽培稻品系‘HD10’与全生育期高感BLS且感病性稳定的籼稻品种‘9311’为亲本,构建了F;定位作图群体,筛选双亲本间多态性分子标记,采用混合分组分析法(BSA)结合SSR和InDel分子标记对群体的苗期和分蘖期抗性位点进行初步定位。分子标记B03021、B03045在双亲及苗期、分蘖期的抗感基因混池之间都存在多态性,并将抗性QTL位点初步定位于第2号染色体分子标记B03021和B03029之间的7 cM区域内,表型贡献率分别为13.1%和17.5%。表明‘HD10’在第2号染色体该区间稳定存在一个主效抗性QTL位点,此位点效应值较大,值得进一步挖掘和利用。
Rice bacterial leaf streak(BLS)is one of the main rice diseases.To exploit major resistance genes/quantitative trait loci(QTLs)of rice to BLS,and provide a basis for enriching rice disease resistant gene resources and breeding resistant cultivars.The F;mapping population was constructed by using a cultivated rice line’HD10’which was highly resistance to BLS and stable resistance at multiple growth stages and an Indica rice variety’9311’which was high susceptible to BLS in the whole growth period as parents.Bulked segregant analysis and SSR/InDel molecular markers were used to map the resistance loci at seedling and tillering stage.The molecular markers B03021 and B03045 were polymorphic between parents and resistant/susceptible genes pools at seedling stage and tillering stage.The resistance loci were initially located in the 7 cM region between the molecular markers B03021 and B03029 on chromosome 2.The phenotypic contribution rates were 13.1%and 17.5%,respectively.Indicating that’HD10’carries a major resistance QTL in this interval on chromosome 2.This QTL has a larger effect value,which should be further explored.
作者
万瑶
罗登杰
何圣贤
覃雪梅
李容柏
刘芳
Wan Yao;Luo Dengjie;He Shengxian;Qin Xuemei;Li Rongbai;Liu Fang(College of Agricultural,Guangxi University,Nanning,530005;State Key Laboratoiy for Protection and Utilization of Subtropical Agro-biore-sources,Guangxi University,Nanning,530005)
出处
《分子植物育种》
CAS
北大核心
2022年第9期2949-2956,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(31460341)
广西自然科学基金项目(2019GXNSFAA185043)
广西创新驱动发展专项(桂科AA17204070)共同资助。
关键词
水稻
细菌性条斑病
抗病性
QTL定位
Rice
Bacterial leaf streak
Disease resistance
QTL mapping
