摘要
目的分析血液标本存放时间延长对生化检验结果的影响。方法选择2020年3月1日—31日在金乡县人民医院进行体检的263名健康体检者作为研究对象,收集受检者的清晨空腹静脉血样本。在采血后1 h、4 h、6 h、8 h、12 h检测血糖(GLU)、γ-谷氨酰转移酶(γ-GT)、丙氨酸转氨酶(ALT)、白蛋白(ALB)、总蛋白(TP)、血尿素氮(BUN)、三酰甘油(TG)、肌酐(Cr)、总胆红素(TBil)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),比较不同时间点上述指标的差异。结果采血4 h后的BUN、TC水平均明显高于采血1 h后,GLU、TBil水平均明显低于采血1 h后〔BUN(mmol/L):7.93±0.96比6.16±1.01,TC(mmol/L):3.59±1.03比3.41±1.01,GLU(μmol/L):7.68±1.02比8.36±1.03,TBil(μmol/L):6.01±1.02比6.29±1.03,均P<0.05〕。采血6 h后的ALT、ALB、TP、BUN、TG水平均明显高于采血1 h后,GLU、TBil、TC水平均明显低于采血1 h后〔ALT(U/L):16.81±1.17比15.49±1.13,ALB(g/L):45.67±1.07比45.37±1.23,TP(g/L):75.34±1.72比72.29±1.23,BUN(mmol/L):7.11±1.04比6.16±1.01,TG(mmol/L):1.27±0.57比1.18±0.42,GLU(μmol/L):6.51±1.07比8.36±1.03,TBil(μmol/L):6.03±1.19比6.29±1.03,TC(mmol/L):3.16±0.61比3.41±1.01,均P<0.05〕。采血8 h、12 h后的ALT、ALB、TP、BUN、TG、Cr、HDL-C、LDL-C水平均明显高于采血1 h后,γ-GT、TBil、TC水平均明显低于采血1 h后〔ALT(U/L):18.59±0.26、18.63±0.17比15.49±1.13,ALB(g/L):47.53±1.21、48.22±1.07比45.37±1.23,TP(g/L):75.21±1.05、75.32±1.21比72.29±1.23,BUN(mmol/L):7.23±0.93、7.46±0.86比6.16±1.01,TG(mmol/L):1.52±0.16、1.37±0.87比1.18±0.42,Cr(μmol/L):90.89±3.32、90.75±3.45比87.86±3.16,HDL-C(mmol/L):1.22±0.15、1.25±0.17比1.17±0.25,LDL-C(mmol/L):2.31±0.42、2.28±0.51比1.96±0.23,γ-GT(U/L):12.79±1.12、12.31±1.05比14.25±1.06,TBil(μmol/L):5.89±1.02、5.78±1.13比6.29±1.03,TC(mmol/L):2.85±0.63、2.65±0.63比1.96±0.23,均P<0.05〕。结论血液标本存放时间的延长会造成生化检验结果部分指标的改变,因此在进行生化检验时,应在采血后尽快实施检验工作,已确保检验结果的准确性,为临床疾病的诊断提供重要的参考依据。
Objective To analyze the influence of blood samples on biochemical test results with the change of storage time.Methods A total of 263 healthy physical examiners in People's Hospital of Jinxiang County from March 1 to March 31,2020 were selected as research objects.The fasting venous blood samples of all subjects were collected in the morning,and after 1 hour,4 hours,6 hours,8 hours and 12 hours of blood collection,the levels of blood glucose(GLU),γ-glutamyl transpeptidase(γ-GT),alanine aminotransferase(ALT),serum albumin(ALB),serum total protein(TP),blood urea nitrogen(BUN),triglyceride(TG),creatinine(Cr),total bilirubin(TBil),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)were measured.The differences of the above indicators at different time points were compared.Results After 4 hours of blood collection,the levels of BUN and TC were higher than those after 1 hour of blood collection,and the levels of GLU and TBil were lower than those after 1 hour of blood collection[BUN(mmol/L):7.93±0.96 vs.6.16±1.01,TC(mmol/L):3.59±1.03 vs.3.41±1.01,GLU(μmol/L):7.68±1.02 vs.8.36±1.03,TBil(μmol/L):6.01±1.02 vs.6.29±1.03,all P<0.05].After 6 hours of blood collection,the levels of ALT,ALB,TP,BUN and TG were higher than those after 1 hour of blood collection,and the levels of GLU,TBil and TC were lower than those 1 hour after blood collection[ALT(U/L):16.81±1.17 vs.15.49±1.13,ALB(g/L):45.67±1.07 vs.45.37±1.23,TP(g/L):75.34±1.72 vs.72.29±1.23,BUN(mmol/L):7.11±1.04 vs.6.16±1.01,TG(mmol/L):1.27±0.57 vs.1.18±0.42,GLU(μmol/L):6.51±1.07 vs.8.36±1.03,TBil(μmol/L):6.03±1.19 vs.6.29±1.03,TC(mmol/L):3.16±0.61 vs.3.41±1.01,all P<0.05].After 8 hours and 12 hous of blood collection,the levels of ALT,ALB,TP,BUN,TG,Cr,HDL-C and LDL-C were higher than those after 1 hour of blood collection,and the levels ofγ-GT,TBil and TC were lower than those after 1 hour of blood collection[ALT(U/L):18.59±0.26,18.63±0.17 vs.15.49±1.13,ALB(g/L):47.53±1.21,48.22±1.07 vs.45.37±1.23,TP(g/L):75.21±1.05,75.32±1.21 vs.72.29±1.23,BUN(mmol/L):7.23±0.93,7.46±0.86 vs.6.16±1.01,TG(mmol/L):1.52±0.16,1.37±0.87 vs.1.18±0.42,Cr(μmol/L):90.89±3.32,90.75±3.45 vs.87.86±3.16,HDL-C(mmol/L):1.22±0.15,1.25±0.17 vs.1.17±0.25,LDL-C(mmol/L):2.31±0.42,2.28±0.51 vs.1.96±0.23,γ-GT(U/L):12.79±1.12,12.31±1.05 vs.14.25±1.06,TBil(μmol/L):5.89±1.02,5.78±1.13 vs.6.29±1.03,TC(mmol/L):2.85±0.63,2.65±0.63 vs.1.96±0.23,all P<0.05].Conclusions The extension of the storage time of blood samples will change some indicators of biochemical test results.Therefore,during biochemical test,the test should be carried out as soon as possible after blood collection to ensure the accuracy of test results and provide important reference for the diagnosis of clinical diseases.
作者
申明霞
Shen Mingxia(Department of Clinical Laboratory,People's Hospital of Jinxiang County,Jining 272200,Shandong,China)
出处
《实用检验医师杂志》
2022年第1期24-27,共4页
Chinese Journal of Clinical Pathologist
关键词
血液标本
存放时间
生化检验
Blood sample
Storage time
Biochemical test
