摘要
目的通过人参bZIP基因家族生物信息学分析,为人参功能基因开发利用提供理论依据。方法运用PlantTFDB数据库预测人参基因组中b ZIP基因;通过ExPASy网站得到人参bZIP基因家族信息和特征;使用MEME网站对PgbZIP基因家族保守基因序列进行分析;运用MEGA软件建立PgbZIP基因家族系统进化关系;利用TBtools软件进行PgbZIP表达分析。结果人参基因组中含有157个bZIP基因家族成员,其中152个定位在细胞核,其余5个分别定位在叶绿体和内质网,相对分子质量在14009.93~83440.38,等电点在4.53~10.05,氨基酸数目在120~760 aa,除PgbZIP157以外,均为亲水蛋白。PgbZIP131在干旱胁迫下的表达量为83.47;PgbZIP99在根和盐胁迫条件下表达量均最高,分别为119.82和117.86;PgbZIP117在叶中表达量为48.54;花中PgbZIP106表达量最高为79.55;成熟果实中表达量最高是PgbZIP118,为119.32;PgbZIP101在未成熟果实中的表达量最高,且为65.32。结论推测PgbZIP99与同源基因AtbZIP11功能相似,可以调控根的分生组织活性;PgbZIP131在干旱胁迫条件下表达量最高,推测PgbZIP131对干旱胁迫有重要调控作用;A亚族成员ABF2/AREB1(AtbZIP36)、ABF3(Atb ZIP37)、ABF4/AREB2(AtbZIP38)参与干旱胁迫和盐胁迫调控,推测与A亚族成员具有同源关系的PgbZIP74、PgbZIP132、PgbZIP122、PgbZIP112在干旱胁迫和盐胁迫条件下具有相似调控作用。
Objective To analyze bZIP gene family bioinformatics of Renshen(Panax ginseng),so as to provide theoretical basis for the development and utilization of ginseng functional genes.Methods Using PlantTFDB database to predict ginseng b ZIP gene;The genetic family information and characteristics of ginseng b ZIP were obtained through ExPASy website;The conserved gene sequences of PgbZIP gene family were analyzed by MEME website;The phylogenetic relationship of PgbZIP gene family were established by using MEGA software;PgbZIP expression analysis were conducted by TBtools software.Results The ginseng genome contained 157 bZIP gene family members,of which 152 were located in the nucleus,and the remaining five were located in the chloroplast and endoplasmic reticulum,respectively.The relative molecular mass was between 14 009.93 and 83 440.38,and the isoelectric point was in the range of 4.53—10.05,the number of amino acids was between 120 aa—760 aa,except for PgbZIP157,all were hydrophilic proteins.The expression level of PgbZIP131 under drought stress was 83.47;the expression level of PgbZIP99 was the highest under root(119.82) and salt stress(117.86) conditions,respectively;the expression level of Pgb ZIP117 in leaves was 48.54;the highest expression level of PgbZIP106 in flowers was 79.55;The highest expression level of Pgb ZIP118 in mature fruits was 119.32;the highest expression level of PgbZIP101 in immature fruits was 65.32.Conclusion It is speculated that PgbZIP99has similar functions to the homologous gene AtbZIP11,and can regulate root meristem activity;PgbZIP131 has the highest expression under drought stress conditions,and it is speculated that PgbZIP131 has an important regulatory effect on drought stress;A subfamily members ABF2/AREB1,ABF3,ABF4/AREB2 is involved in the regulation of drought stress and salt stress.It is speculated that PgbZIP74,PgbZIP132,PgbZIP122 and PgbZIP112,which have a homologous relationship with members of subfamily A,have similar regulatory effects under drought stress and salt stress.
作者
王思嘉
孙嘉莹
刘美琦
任伟超
于欣欣
刘秀波
马伟
WANG Si-jia;SUN Jia-ying;LIU Mei-qi;REN Wei-chao;YU Xin-xin;LIU Xiu-bo;MA Wei(College of Pharmaceutical Sciences,Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Jiamusi,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China;Key Laboratory of Basic and Application Research of Beiyao(Heilongjiang University of Chinese Medicine),Ministry of Education,Harbin 150040,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第9期2786-2794,共9页
Chinese Traditional and Herbal Drugs
基金
黑龙江省“头雁”团队项目(黑龙江省头雁行动领导小组文件[2019] 5号)
黑龙江中医药大学科研基金项目(中药健康相关产品研发及产业化专项)(2019BJP06)。
关键词
人参
BZIP
生物信息学
基因家族
盐胁迫
干旱胁迫
Panax ginseng C.A.Meyer
b ZIP
bioinformatics
gene family
salt stress
drought stress
