摘要
目的建立一种同时测定金银花Lonicerae Japonicae Flos 20种黄酮类成分含量的方法,并对含量结果进行多元统计分析,探讨不同种质金银花发育过程中黄酮类成分的含量变化规律,为优良品种选育与金银花药材质量控制提供技术支撑。方法建立基于HPLC-ESI-MS/MS同时测定金银花20种黄酮类成分含量的方法,测定5种种质金银花不同发育时期黄酮类成分含量,利用主成分分析(principal components analysis,PCA)和正交偏最小二乘判别分析(orthogonal partial least-squares discrimination analysis,OPLS-DA)研究其变化规律,并筛选差异性活性成分。结果建立的HPLC-ESI-MS/MS方法重复性好、稳定性好,5种种质金银花总黄酮含量:华金6号>九丰一号>四季花>红白忍冬>华金3号,木犀草苷含量:华金6号>红白忍冬>九丰一号>四季花>华金3号;在各种质金银花发育过程中,木犀草苷和总黄酮含量在二白期或大白期最高;通过多元统计分析确定了不同种质间存在9个潜在差异性黄酮标志物(野漆树苷、似梨木双黄酮、忍冬苷、水仙苷、芦丁、异鼠李素-3-O-葡萄糖苷、圣草酚-7-O-葡萄糖苷、芹菜素和山柰酚-3-O-芸香糖苷),不同发育时期有7个差异性黄酮标志物(苜蓿素、野漆树苷、香叶木素、芹菜素-7-O-葡萄糖苷、金圣草黄素、紫云英苷和圣草酚-7-O-葡萄糖苷)。结论金银花黄酮类成分组成与含量既决定于种质又受发育程度的影响,选育优良品种、确定合理采收时期是提高金银花药材黄酮类成分含量的有效途径。
Objective To establish a method for simultaneous determination of 20 flavonoids in Lonicerae Japonicae Flos(LJF),and multivariate statistical analysis of the content results was carried out to explore the content changes of flavonoids in different germplasm during the development of LJF,providing technical support for the breeding of superior varieties and quality control of LJF.Methods To establish a method for simultaneous determination of 20 flavonoids in LJF based on HPLC-ESI-MS/MS,and to determine the content of flavonoids in five germplasm LJF at different development stages.Principal components analysis(PCA)and orthogonal partial least-squares discrimination analysis(OPLS-DA)were used to analyze the change rule,and the different active components were screened.Results The established HPLC-ESI-MS/MS method has good repeatability and stability.The contents of total flavonoids in LJF of five germplasms were as follows:“Huajin No.6”>“Jiufeng No.1”>“Sijihua”>“Hongbairendong”>“Huajin No.3”,and luteoloside content was as follows:“Huajin No.6”>“Hongbairendong”>“Jiufeng No.1”>“Sijihua”>“Huajin No.3”;The contents of luteoloside and total flavonoids were the highest in the second white stage or big white stage.Through multivariate statistical analysis,it was determined that there were nine potential differential flavonoid markers(rhoifolin,ochnaflavone,lonicerin,narcissoside,rutin,isorhamnetin-3-O-glucoside,eriodictyol-7-O-glucoside,apigenin,and kaempferol-3-O-rutinoside)among different germplasm and seven differential flavonoid markers(tricin,rhoifolin,diosmetin,apigenin-7-O-glucoside,chrysoeriol,astragaloside,and eriodictyol-7-O-glucoside)at different development stages.Conclusion The composition and content of flavonoids in LJF are determined by germplasm and development period.Breeding excellent varieties and determining reasonable harvest time are effective ways to improve the content of flavonoids in LJF.
作者
黄文静
熊乐文
张龙霏
张芳
韩翔宇
张永清
张利
杨寒冰
HUANG Wen-jing;XIONG Le-wen;ZHANG Long-fei;ZHANG Fang;HAN Xiang-yu;ZHANG Yong-qing;ZHANG Li;YANG Han-bing(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Provincial Key Laboratory of Traditional Chinese Medicine Resources,Jinan 250355,China;Shandong Zhongping Pharmaceutical Co.Ltd.,Pingyi 273300,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第10期3156-3164,共9页
Chinese Traditional and Herbal Drugs
基金
山东省重大科技创新工程项目(2019JZZY011020)
山东省高校中药质量控制与全产业链建设协同创新中心(CYLXTCX2020)
国家重点研发计划项目(2017YFC1701503)
山东省重点研发计划“中医经方精准化关键技术示范研究”课题(2016CYJS08A01)
国家自然科学基金资助项目(81903750)。
