摘要
目的探讨AMD3100(Plerixafor,普乐沙福)干预CXCR4对哮喘小鼠气道黏蛋白MUC5ac的影响及可能机制。方法6~8周BALB/C雌性小鼠分为正常组(NS组)、哮喘组(AS组)和CXCR4阻滞剂AMD3100组(AMD组),各8只。卵清蛋白(OVA)致敏后,雾化吸入OVA激发制备哮喘模型,各组在激发前进行干预。通过酶联免疫吸附法检测肺泡灌洗液中白细胞介素(IL)-4,IL-5及CXCR4,肺组织行HE染色、AB-PAS染色观察肺组织病理学改变;免疫组织化学检测CXCR4、气道MUC5ac蛋白表达变化,RTPCR检测肺组织CXCR4 mRNA表达水平。结果AMD3100组小鼠BALF中IL-4、IL-5、CXCR4水平、支气管周围炎症病理评分、气道上皮杯状细胞和黏液物质阳性相对着色面积、肺组织MUC5ac蛋白、CXCR4蛋白表达及CXCR4 mRNA表达水平较哮喘组降低,但仍高于正常对照组(P<0.05)。结论AMD3100干预CXCR4蛋白的表达,抑制哮喘小鼠气道炎症反应及下调MUC5ac的表达,减轻气道黏液高分泌,改善哮喘症状。
Objective To explore the effect and mechanism of AMD3100 on CXCR4 and MUC5 ac in asthma mice.Methods Female BALB/c mice aged to 6~8 weeks were randomly divided into normal group(NS group),asthma group(AS group),CXCR4 blockers group(AMD group),Eight in each group.Asthma was induced by OVA sensitization and challenge,intervention was performed in each group before stimulation.IL-4,IL-5,CXCR4 in BALF were detected by ELISA.The pathological changes of lung were observed by HE staining,AB-PAS staining;IHC and RT-PCR were used to detect the expression of Muc5 ac,CXCR4 protein level and CXCR4 mRNA in lung tissues.Results The CXCR4,IL-4 and IL-5 levels,infiltration of inflammatory cells around the airway of lung tissue,positive relative coloring area of airway mucus,CXCR4 and Muc5 ac protein content,mRNA relative expression of CXCR4,AMD group were lower than AS group(P<0.01).was higher than NS group(P<0.05).Conclusions AMD3100 intervention can reduce the expression of CXCR4 protein,inhibits airway inflammatory and down regulates the expression of MUC5 AC in asthmatic mice,alleviate airway mucus hypersecretion,and improves asthmatic symptoms.
作者
王莉
刘小静
张建勇
WANG Li;LIU Xiaojing;ZHANG Jianyong(Department of Respiratory and Critical Care Medicine,Affliated Hospital of Zunyi Medical University,Zunyi 563000,China)
出处
《实用医学杂志》
CAS
北大核心
2022年第9期1082-1087,共6页
The Journal of Practical Medicine
基金
贵州省科技厅资助项目(编号:黔科合支撑[2020]4Y162号)
遵义市科技厅(编号:遵市科合HZ字(2019)116号)。
