摘要
目的:比较不同产地苍术脂溶性成分在含量和组成上的差异,为苍术药材建立科学合理的产地鉴别和质量评价提供试验依据。方法:采用GC⁃MS法,DB⁃5 MS色谱柱(30 m×0.25 mm,0.25 mm),载气He,流速1.0 ml·min^(-1),进样口温度250℃,分流比20∶1,离子源温度230℃,电离方式EI;同时采用SPSS软件进行聚类分析。结果:不同产地3种苍术的脂溶性成分检测出20个共有成分,包括佛术烯、α⁃芹子烯、5⁃异丙烯基⁃3,6⁃二甲基⁃6⁃乙烯基⁃4,5,6,7⁃四氢⁃1⁃香豆酮和β⁃桉叶醇等。5⁃异丙烯基⁃3,6⁃二甲基⁃6⁃乙烯基⁃4,5,6,7⁃四氢⁃1⁃香豆酮相对含量最高,β⁃桉叶醇次之。茅苍术中α⁃芹子烯的相对含量明显低于北苍术和关苍术(P<0.05);关苍术和茅苍术中佛术烯含量均明显高于北苍术(P<0.01);5⁃异丙烯基⁃3,6⁃二甲基⁃6⁃乙烯基⁃4,5,6,7⁃四氢⁃1⁃香豆酮的相对含量北苍术和茅苍术之间有明显差异(P<0.05);北苍术和茅苍术的β⁃桉叶醇含量高于关苍术(P<0.01)。聚类分析将不同产地苍术划分为3个表征群,与茅苍术、北苍术和关苍术分类基本一致。结论:关苍术与茅苍术、北苍术特征图谱差异明显,通过不同产地不同品种4个成分分析并结合聚类分析,反映药材的道地性,为苍术药材质量标准优化、市场规范性以及临床疗效提供了科学的依据。
Objective:To compare different origin of liposoluble components of Atractylodis Rhizoma from different producing area and to provide experimental basis for scientific identification and quality evaluation of Atractylodis Rhizoma.Methods:The analysis was using GC⁃MS carried on a DB⁃5 column(30 m×0.25 mm,0.25 mm),carried gas He at the flow rate of 1.0 ml·min^(-1).The inter temperature was set at 250℃,the split ratio about 20∶1 and the ion source temperature was sat at 230℃.The ionization methods EI.At the same time,SPSS software was used for cluster analysis.Results:20 common components were detected in lipid soluble compo⁃nents of three kinds of Atractylodis Rhizoma from different areas,including eremophilene,α⁃selinene,5⁃isopropenyl⁃3,6⁃dimethyl⁃6⁃vinyl⁃4,5,6,7⁃tetrahydro⁃1⁃benzofuran,β⁃eudesmol and so on.The relative content of 5⁃isopropenyl⁃3,6⁃dimethyl⁃6⁃vinyl⁃4,5,6,7⁃tet⁃rahydro⁃1⁃benzofuran was the highest,followed byβ⁃eudesmol.The relative content ofα⁃selinene in Atractylodes lancea was signifi⁃cantly lower than that in Atractylodes chinensis and Atractylodes japonica(P<0.05).The content of eremophilene in Atractylodes ja⁃ponica and Atractylodes lancea was significantly higher than that in Atractylodes chinensis(P<0.01).The relative content of 5⁃isopro⁃penyl⁃3,6⁃dimethyl⁃6⁃vinyl⁃4,5,6,7⁃tetrahydro⁃1⁃benzofuran was significantly different between Atractylodes chinensis and Atractylodes japonica(P<0.05).The content ofβ⁃eudesmol from Atractylodes chinensis and Atractylodes lancea was higher than that of Atractylodes japonica(P<0.01).Cluster analysis divided Atractylodes Rhizoma from different habitats into three characterization groups,which were basically consistent with the classification of Atractylodes lancea,Atractylodes japonica and Atractylodes chinensis.Conclusion:The characteristic map is significantly different from three kinds of Atractylodis Rhizoma.Through the analyses of four components of different kinds of Atractylodis Rhizoma from different areas and cluster analysis,the geoherbaalism of herbal medicines is reflected,which provide scientific basis for the optimization of the quality standard,market standardization and clinical efficacy of Atractylodes Rhizoma.
作者
林雅丽
江阳超
袁文钦
钟瑞
陈乃宏
刘应蛟
Lin Yali;Jiang Yangchao;Yuan Wenqin;Zhong Rui;Chen Naihong;Liu Yingjiao(School of Pharmacy,Jiangxi University of Chinese Medicine,Jiangxi Nanchang 330004,China;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College)
出处
《中国药师》
CAS
2022年第5期911-917,共7页
China Pharmacist
基金
江西省中医药管理局科技计划项目(编号:2021A333)
江西省卫生健康委科技计划项目(编号:202211412)
博士启动资金项目(编号:2021BSZR012)
国家自然科学基金(编号:82060710)。
关键词
苍术
脂溶性成分
气相色谱⁃质谱
产地
主成分分析
Atractylodes Rhizoma
Liposoluble components
GC⁃MS
Producing area
Principal component analysis
