摘要
目的 研究胡芦巴总皂苷(TFGs)缓解小鼠疲劳的药效。方法 取5批BALB/c小鼠(每批75只),随机分为对照组(纯水20 mL·kg^(-1))、阳性药组(西洋参含片625.0 mg·kg^(-1))和低、中、高剂量TFGs组(65, 130,260 mg·kg^(-1)TFGs),每组15只,灌胃给药30 d后,对每批小鼠以不同的处理方式检测相关药效学指标,并运用网络药理学预测潜在作用机制。结果 对照组,低、中、高剂量TFGs组和阳性药组小鼠血乳酸曲线下面积(比值)分别为(264.77±41.26),(247.81±28.37),(210.60±34.46),(225.57±42.11),(224.27±22.00),血清尿素氮水平分别为(9.64±1.05),(8.70±1.22),(7.33±0.86),(7.43±1.07),(7.88±1.14)mmol·L^(-1),丙二醛水平分别为(9.06±2.03),(7.24±1.67),(7.04±1.29),(6.60±2.06),(7.04±2.10)nmol·mg^(-1)port,肌糖原水平分别为(0.72±0.10),(0.89±0.32),(1.07±0.24),(1.26±0.31),(1.02±0.15)mg·g^(-1),超氧化物歧化酶水平分别为(106.54±32.73),(126.10±20.89),(135.78±27.44),(139.39±18.87),(127.67±30.43)U·mg^(-1)port,谷胱甘肽过氧化物酶分别为(189.03±59.27),(245.62±56.54),(296.02±54.68),(264.58±57.42),(287.33±85.53)U·mg^(-1)port,总抗氧化能力水平分别为(5.27±2.01),(6.49±1.78),(9.33±2.26),(8.05±2.15),(9.95±2.45)U·mg^(-1)port,肝糖原水平分别为(6.44±1.21),(6.07±1.94),(8.33±3.09),(8.30±1.69),(8.74±2.48)mg·g^(-1)。与对照组比较,中、高剂量TFGs组显著延长小鼠负重游泳时间,且降低血乳酸、血清尿素氮、丙二醛水平,提高肌糖原、超氧化物歧化酶、谷胱甘肽过氧化物酶和总抗氧化能力水平,差异均有统计学意义(P<0.05,P<0.01),肝糖原水平无统计学意义,有效缓解小鼠疲劳症状。胡芦巴中有12个潜在活性成分,主要功效物质为皂苷类,作用机制与80个基因,多条氧化应激通路相关。结论 TFGs有缓解疲劳的药效,作用机制与抗氧化密切相关。
Objective To explore the efficacy of Trigonella foenum greacum L Saponin(TFGs) in relieving fatigue in mice. Methods Five batches of BALB/c mice(75 mice each batch) were randomly divided into control group(pure water, 20.0 mL·kg^(-1)), positive drug group(Panax Quinquefolius Buccal tablet 625.0 mg·kg^(-1)), TFGs-L, TFGs-M, TFGs-H groups(65, 130, 260 mg·kg^(-1)TFGs) and 15 rats per group. After 30 days of intragastric administration, each batch of mice was treated with different methods to detect relevant pharmacodynamic indicators, and network pharmacology was used to predict the potential mechanism of action. Results The area under the blood lactate curve in control group,TFGs-L,TFGs-M,TFGs-H groups and positive drug group were( 264. 77 ± 41. 26),( 247. 81 ± 28. 37),( 210. 60 ± 34. 46),( 225. 57 ± 42. 11),( 224. 27 ± 22. 00);the level of serum urea nitrogen were( 9. 64 ± 1. 05),( 8. 70 ± 1. 22),( 7. 33 ± 0. 86),( 7. 43 ± 1. 07),( 7. 88 ± 1. 14) mmol·L^(-1),the level of malondialdehyde were( 9. 06 ± 2. 03),( 7. 24 ± 1. 67),( 7. 04 ± 1. 29),( 6. 60 ± 2. 06),( 7. 04 ± 2. 10) nmol·mg^(-1)port;the level of muscle glycogen were( 0. 72 ± 0. 10),( 0. 89 ± 0. 32),( 1. 07 ± 0. 24),( 1. 26 ± 0. 31),( 1. 02 ± 0. 15) mg·g^(-1);the level of superoxide dismutase were( 106. 54 ± 32. 73),( 126. 10 ± 20. 89),( 135. 78 ± 27. 44),( 139. 39 ± 18. 87),( 127. 67 ± 30. 43)U·mg^(-1)port;the level of glutathione peroxidase were( 189. 03 ± 59. 27),( 245. 62 ± 56. 54),( 296. 02 ± 54. 68),( 264. 58 ± 57. 42),( 287. 33 ± 85. 53) U·mg^(-1)port;the level of total antioxidant ability were( 5. 27 ± 2. 01),( 6. 49 ± 1. 78),( 9. 33 ± 2. 26),( 8. 05 ± 2. 15),( 9. 95 ± 2. 45) U·mg^(-1)port;the level of liver glycogen were( 6. 44 ± 1. 21),( 6. 07 ± 1. 94),( 8. 33 ± 3. 09),( 8. 30 ± 1. 69),( 8. 74 ± 2. 48) mg·g^(-1). Compared with control group,the TFGs-M,TFGs-H groups could significantly prolonged the loading swimming time of mice,and reduced blood lactic acid,serum urea nitrogen,and malondialdehyde levels,and increased muscle glycogen,superoxide dismutase,glutathione peroxidase and total antioxidant ability level( P < 0. 05,P < 0. 01),maintain liver glycogen level,effectively alleviate fatigue symptoms in mice. Fenugreek contains 12 potential active ingredients,the main functional substance is saponins,and the mechanism of action is related to 80 genes and multiple oxidative stress pathways. Conclusion TFGs can relieve fatigue,and the mechanism of action is closely related to antioxidant.
作者
闫冬
储金林
马睿瑶
毛新民
李琳琳
YAN Dong;CHU Jin-lin;MA Rui-yao;MAO Xin-min;LI Lin-lin(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghar Autonomous Region,China;College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghar Autonomous Region,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2022年第9期974-978,共5页
The Chinese Journal of Clinical Pharmacology
基金
新疆维吾尔自治区自然科学基金面上基金资助项目(2018D01C171)。
关键词
胡芦巴
抗疲劳
抗氧化
网络药理学
Fenugreek
anti-fatigue
anti-oxidation
network pharmacology
