摘要
为提高红掌胚芽愈伤组织诱导率、不定芽的分化率及壮苗生根率,本研究以‘云芝’红掌自交系F_(1)代种子为外植体,对种胚愈伤诱导及不定芽增殖的培养基配方进行添加不同质量浓度6-BA、NAA和IBA的影响试验;对单芽壮苗生根的培养基配方进行添加不同质量浓度NAA和IBA的影响试验。结果显示,诱导种胚愈伤的最佳培养基为1/2MS+0.5 mg/L NAA+1.5 mg/L 6-BA,诱导率达89.17%;诱导不定芽增殖的最佳培养基为1/2MS+0.1 mg/L NAA+0.2 mg/L IBA+1.0 mg/L 6-BA,增殖系数为2.79;单芽壮苗生根的最佳培养基为1/2MS+0.4 mg/L NAA+0.2 mg/L IBA,生根率达100%,壮苗指数为16.70,组培苗品质最好。本研究结果可为红掌组培快繁技术提供参考。
To improve the induction rate of embryogenic callus,the differentiation efficiency and seedling rooting rate of adventitious bud in the embryo of Anthurium andraeanum,the effects of different medium were investigated,using the seeds of Anthurium andraeanum inbred F_(1) line‘Yunzhi’as explants.In this study,medium containing different concentrations of 6-BA,NAA,and IBA were tested.The results showed that the optimal medium to improve embryogenic callus induction rate was 1/2MS containing 0.5 mg/L NAA and 1.5 mg/L 6-BA,which could reached an induction rate of 89.17%.For increasing adventitious bud proliferation,the optimum medium was1/2MS containing 0.1 mg/L NAA,0.2 mg/L IBA,and 1.0 mg/L 6-BA,and the proliferation coefficient was 2.79.The optimal medium for improving rooting of single bud seedling was 1/2MS with 0.4 mg/L NAA and 0.2 mg/L IBA,and the rooting rate reached 100%.In addition,the strong seedling index was 16.70,as well as the best quality of tissue-cultured seedlings was obtained.This study could provide a technical guidance for in vitro rapid propagation of Anthurium andraeanum.
作者
姚凤琴
林发壮
钟琳珊
陈昌铭
郭芸玮
安慧珍
Yao Fengqin;Lin Fazhuang;Zhong Linshan;Chen Changming;Guo Yunwei;An Huizhen(Sanming Academy of Agricultural Sciences,Shaxian,365509;Xiamen Agricultural Technology Extension Center,Xiamen.361009)
出处
《分子植物育种》
CAS
北大核心
2022年第10期3313-3318,共6页
Molecular Plant Breeding
基金
福建省引导性项目(2019N0044)
福建省星火项目(2020S0017)
三明市科技计划项目(2020-N-4)共同资助。
关键词
红掌
愈伤诱导
不定芽
增殖
壮苗生根
Anthurium andraeanum
Callus induction
Adventitious bud
Proliferation
Seedling rooting
