摘要
目的研究不同碘营养对哺乳期大鼠及子代甲状腺功能的影响,为孕期科学补碘及甲状腺疾病筛查提供依据。方法选择断乳2周SPF级Wistar大鼠180只,按随机数字表法,以碘摄入量为0.00、1.50、5.50、70.00和350.00μg/L分5组,饮用含碘化钾去离子水干预3个月交配受孕。每组取15只妊娠大鼠并随机留存每只孕鼠3~4只子代大鼠;继续干预21天,取哺乳期大鼠及子代主动脉血并分离血清检测甲状腺功能指标。结果动物模型建立成功,各组大鼠尿碘含量比较,差异有统计学意义(尿碘中位数分别为3.54、51.41、286.80、644.19、2368.70μg/L,P<0.01)。哺乳期各组大鼠TgAb、TPOAb和双抗体阳性率比较,差异有统计学意义(P<0.05);MID组、SID组TgAb阳性率明显高于NI组(P<0.05);MID组TPOAb阳性率明显高于NI组(P<0.05);MID组、MIE组双抗体阳性率明显高于NI组(P<0.05)。子代大鼠:各组间TSH、FT 4、FT 3、TgAb、TPOAb和双抗体阳性率比较,差异有统计学意义(P<0.05);SIE组、MIE组、MID组的TSH均与NI组比较,差异有统计学意义(P<0.05);SID组FT 4水平与NI组比较,差异有统计学意义(P<0.05);MIE组、MID组、SID组FT 3水平与NI组比较,差异有统计学意义(P<0.05);SID组TgAb阳性率与NI组比较,差异有统计学意义(P<0.05);MID组、SID组TPOAb阳性率与NI组比较,差异有统计学意义(P<0.05);SID组双抗体阳性率高于NI组(P<0.05)。SID组亲代和子代大鼠TSH间呈正相关;MID组亲代大鼠FT 3与子代大鼠FT 4、FT 3、ATPO呈正相关;NI组亲代大鼠ATG、ATPO与子代大鼠的FT 4呈正相关;MIE组:亲代大鼠FT 3与子代ATG呈正相关;亲代大鼠ATG与子代ATG呈负相关;SIE组:亲代大鼠ATG与子代TSH呈正相关,而亲代大鼠ATPO与子代ATG呈负相关;以上相关分析结果比较,差异均有统计学意义(P均<0.05)。结论碘缺乏和碘过量均可导致哺乳期大鼠及子代甲状腺激素水平发生改变,会引起亲代大鼠及其子代的甲状腺相关抗体阳性率升高。当碘缺乏及正常摄入时,亲代与子代大鼠的某几个甲状腺功能指标间呈正相关。当碘过量摄入时,亲代与子代大鼠不同甲状腺功能指标间均呈负相关。
Objective To study the effects of different iodine nutrition on thyroid function of lactating rats and their offspring,and to provide basis for scientific iodine supplement and thyroid disease screening during pregnancy.Methods A total of 180 SPF Wistar rats were randomly divided into 5 groups with iodine intake of 0.00,1.50,5.50,70.00 and 350.00μg/L according to the random number table method.Fifteen pregnant rats were selected from each group and 3-4 offspring were randomly retained from each pregnant rat.After 21 days of intervention,aortic blood was collected from lactating rats and their offspring,and serum was separated to detect thyroid function indexes.Results The animal model was established successfully,and there was statistically significant difference in urinary iodine content among all groups(median of urinary iodine was 3.54,51.41,286.80,644.19,2368.70μg/L,P<0.01).The positive rates of TgAb,TPOAb and double antibody in lactation rats were statistically significant(P<0.05).The positive rate of TgAb in MID group and SID group was significantly higher than that in NI group(P<0.05).The positive rate of TPOAb in MID group was significantly higher than that in NI group(P<0.05).The positive rate of double antibody in MID and MIE groups was significantly higher than that in NI group(P<0.05).For offspring rats,the positive rates of TSH,FT 4,FT 3,TgAb,TPOAb and double antibody were statistically different among all groups(P<0.05).In TSH of SIE group,MIE group and MID group was significantly different from that of NI group(P<0.05).The level of FT 4 in SID group was significantly different from that in NI group(P<0.05).The FT 3 level of MIE group,MID group and SID group was significantly different from that of NI group(P<0.05).The positive rate of TgAb in SID group was significantly different from that in NI group(P<0.05).The positive rate of TPOAb in MID group and SID group was significantly different from that in NI group(P<0.05).The positive rate of double antibody in SID group was higher than that in NI group(P<0.05).There was a positive correlation between TSH of parental and offspring rats in SID group.There was a positive correlation between parental FT 3 and offspring FT 4,FT 3 and ATPO in MID group.ATG and ATPO in NI group were positively correlated with FT 4 in offspring rats.In MIE group,There had a positive correlation between parental FT 3 and progeny ATG.There was a negative correlation between ATG of parental rats and ATG of offspring.In SIE group,ATG of parental rats was positively correlated with TSH of offspring,while ATPO was negatively correlated with ATG of offspring.The above correlation analysis results showed statistical differences(P<0.05).Conclusion Both iodine deficiency and iodine overdose can change the thyroid hormone levels of lactating rats and their offspring,and increase the positive rate of thyroid related antibodies in parental rats and their offspring.When iodine deficiency and normal intaking,there was a positive correlation between some thyroid function indexes of parental rats and offspring rats.There was a negative correlation between different thyroid function indexes of parental and offspring rats when iodine overdose was ingested.
作者
祝宇铭
王琛琛
林勤
黄佳
ZHU Yuming;WANG Chenchen;LIN Qin(Institute of Environmental Health and Endemic Disease Control,Xinjiang Center for Disease Control and Prevention,Xinjiang 830002,China)
出处
《医学研究杂志》
2022年第6期51-55,60,共6页
Journal of Medical Research
基金
新疆维吾尔自治区公益性科研项目(ky2021113)。
关键词
碘营养
甲状腺激素
哺乳期大鼠
Iodine nutrition
Thyroid hormone
Lactating rats
