摘要
【目的】探讨表油菜素内酯(EBR)和过氧化氢(H_(2)O_(2))对低温胁迫后烟草幼苗生理和生长特性的影响。【方法】以烤烟品种K326为材料,研究低温胁迫后喷施外源表油菜素内酯(EBR,0.01 mg/L)和过氧化氢(H_(2)O_(2),340 mg/L)对烤烟苗期抗氧化酶(SOD、POD、CAT)活性、渗透调节物质、丙二醛(MDA)和生物量的影响。【结果】(1)烟草苗期低温胁迫(CK1)4 d与正常生长(CK2)烟株相比SOD活性降低,POD、CAT活性升高,MDA、可溶性糖和脯氨酸含量上升,可溶性蛋白含量下降,生物量降低;(2)低温胁迫结束后,喷施外源EBR(T1)和H_(2)O_(2)(T2)相比低温对照(CK1),恢复生长12 d烟苗SOD、POD、CAT活性分别提高61.87%和47.37%、239.06%和193.11%、168.07%和139.63%,MDA含量分别降低60.00%和50.00%,渗透调节物质含量和生物量显著增加;(3)喷施外源EBR(T1)和H_(2)O_(2)(T2)与正常生长(CK2)烟株相比,在恢复生长8 d和12 d时SOD、POD、CAT活性与可溶性糖含量无显著差异,MDA含量降低,脯氨酸含量显著上升。【结论】喷施外源EBR(0.01 mg/L)和H_(2)O_(2)(340 mg/L)能加快低温胁迫后烤烟K326幼苗的生理和生长恢复进程。
[Objective]This study aims to explore the effects of epibrassinolide and hydrogen peroxide on physiological and growth characteristics of tobacco seedlings under low temperature stress.[Methods]Using flue-cured tobacco variety K326 as material, the effects of exogenous epibrassinolide(EBR, 0.01 mg/L) and hydrogen peroxide(H_(2)O_(2), 340 mg/L) on antioxidant enzymes activity(SOD, POD,CAT), osmotic regulation substances, malondialdehyde(MDA) and biomass of flue-cured tobacco at seedling stage were studied.[Results](1) Compared with normal growth(CK2), SOD activity decreased, POD and CAT activity increased, MDA, soluble sugar and proline content increased, soluble protein content decreased, and biomass decreased in tobacco seedlings under low temperature stress(CK1) at 4 d.(2) After the end of low temperature stress, the SOD, POD and CAT activities of tobacco seedlings sprayed with exogenous EBR(T1) and H_(2)O_(2)(T2) increased by 61.87% and 47.37%, 239.06% and 193.11%, 168.07% and 139.63%, respectively as compared with the control(CK1). MDA content decreased by 60.00% and 50.00%, respectively, while osmoregulation substances contents and biomass increased significantly.(3) Compared with normal growth(CK2), the tobacco plants sprayed with exogenous EBR(T1) and H_(2)O_(2)(T2) had no significant differences in SOD, POD, CAT activity and soluble sugar content at 8 days and 12 days after resuming growth, but exhibited decreased MDA content and significantly increased proline content.[Conclusion]Spraying exogenous EBR(0.01 mg/L) and H_(2)O_(2)(340mg/L) can accelerate the physiological and growth recovery process of flue-cured tobacco K326 seedlings under low temperature stress.
作者
许金亮
谢鹏飞
向世鹏
范敏
陈治峰
沈子奇
张芸萍
李强
XU Jinliang;XIE Pengfei;XIANG Shipeng;FAN Min;CHEN Zhifeng;SHEN Ziqi;ZHANG Yunping;LI Qiang(College of Agronomy,Hunan Agriculural University,410128;Changsha Branch of Hunan Provincial Tobacco Company,410001)
出处
《中国烟草学报》
CAS
CSCD
北大核心
2022年第3期44-51,共8页
Acta Tabacaria Sinica
基金
湖南省烟草公司长沙市烟草公司项目(20-24A01)。
