摘要
目的 探讨血清微小RNA(miRNA)-196a、miRNA-92a表达水平与宫颈癌根治术后复发的关系。方法 选取2015年1月至2016年6月甘肃省肿瘤医院收治的113例行宫颈癌根治术患者为宫颈癌组,68名体检健康女性为对照组,qRT-PCR检测两组血清miRNA-196a、miRNA-92a表达。分析宫颈癌患者血清miRNA-196a、miRNA-92a水平与临床病理特征的关系。术后随访5年,根据血清miRNA-196a、miRNA-92a水平均值将宫颈癌患者分为高表达和低表达。比较高表达和低表达宫颈癌患者复发率。多因素Cox回归分析宫颈癌根治术后复发的影响因素。受试者操作特征(ROC)曲线分析血清miRNA-196a、miRNA-92a水平对宫颈癌根治术后复发的预测价值。结果 宫颈癌组血清miRNA-196a、miRNA-92a表达高于对照组,差异有统计学意义(P <0.05)。不同国际妇产科联盟(FIGO)分期、间质浸润深度、淋巴结转移的宫颈癌患者血清miRNA-196a、miRNA-92a水平比较,差异有统计学意义(P <0.05)。截至末次随访,113例宫颈癌患者术后复发25例(22.12%),miRNA-196a、miRNA-92a高表达复发率高于低表达,差异有统计学意义(P <0.05)。FIGO分期ⅡA期、间质浸润深度≥1/2、淋巴结转移、miRNA-196a≥5.239、miRNA-92a≥1.816是宫颈癌根治术后复发的独立危险因素(HR> 1,P <0.05)。ROC曲线显示,miRNA-196a联合miRNA-92a预测宫颈癌根治术后复发的曲线下面积大于两者单独预测(Z=2.319、2.582,P=0.020、0.010)。结论 宫颈癌血清miRNA-196a、miRNA-92a水平高表达,二者与FIGO分期、间质浸润深度、淋巴结转移、复发有关,可作为宫颈癌根治术后复发的预测指标。
Objective To investigate the relationship between the expression levels of serum microRNA(miRNA)-196a and miRNA-92a and recurrence of cervical cancer after radical surgery. Methods A total of 113 patients undergoing radical resection of cervical cancer who were admitted to Gansu Provincial Cancer Hospital from January 2015 to June2016 were selected as the cervical cancer group, qRT-PCR was used to detect serum miRNA-196a and miRNA-92a levels in the both groups. The relationship between serum miRNA-196a and miRNA-92a levels and clinicopathological characteristics of cervical cancer patients was analyzed. Post-operative follow-up for five years, and the cervical cancer patients were divided into high expression and low expression according to the mean value of serum miRNA-196a and miRNA-92a. The recurrence rates of cervical cancer patients in high expression and low expression were compared,multivariate Cox regression was used to analyze the influencing factors of cervical cancer after radical surgery. Receiver operator characteristic(ROC) curve was used to analyze the predictive value of serum miRNA-196a and miRNA-92a levels on recurrence of cervical cancer after radical surgery. Results The expression levels of serum miRNA-196a and miRNA-92a in cervical cancer group were higher than those in control group, and the differences were statistically significant(P < 0.05). There were statistically significant differences in serum miRNA-196a and miRNA-92a levels among cervical cancer patients with different Federation of Gynecology and Obstetrics(FIGO) stages,interstitial invasion depth, and lymph node metastasis (P < 0.05). By the last follow-up, 25 patients(22.12%) of 113 cervical cancer patients had recurrence after surgery and the recurrence rate of miRNA-196a and miRNA-92a of the high expression were higher than those of the low expression,and the differences were statistically significant(P < 0.05). FIGO stage ⅡA, interstitial invasion depth ≥1/2, lymph node metastasis, miRNA-196a ≥5.239, and miRNA-92a ≥1.816 were the independent risk factors for recurrence on cervical cancer after radical surgery(HR > 1, P < 0.05). ROC curve showed that the area under the curve of miRNA-196a combined with miRNA-92a in predicting the recurrence on cervical cancer after radical surgery was greater than that of both alone(Z = 2.319, 2.582, P = 0.020, 0.010). Conclusion Serum miRNA-196a and miRNA-92a levels in cervical cancer are high expression, both of which are associated with FIGO stage, depth of interstitial infiltration, lymph node metastasis, and recurrence, and can be used as predictors of recurrence on cervical cancer after radical surgery.
作者
王雨村
胡拓阳
崔静
韩萍
丁燕
WANG Yucun;HU Tuoyang;CUI Jing;HAN Ping;DING Yan(The Second Department of Gynecology and Oncology,Gansu Provincial Cancer Hospital,Gansu Province,Lanzhou 730050,China;Department of Radiology,Gansu Provincial Cancer Hospital,Gansu Province,Lanzhou 730050,China)
出处
《中国医药导报》
CAS
2022年第17期27-31,共5页
China Medical Herald
基金
甘肃省卫生健康行业科研项目(GSWSKY2020-23)。
