摘要
多糖是白芨块茎中含量最多的物质,水提醇沉法是白芨多糖最简单有效的提取方法。该研究在单因素试验的基础上,以液料比、提取时间和提取温度为影响因素,以多糖提取率为响应面值,对白芨多糖水提醇沉工艺进行优化,并考察了放置时间、剪切速率、加热温度、耐盐性等因素对白芨多糖黏度的影响。结果表明,最优提取条件为料液比为1∶59.77,提取时间为1.42 h,提取温度为67.79℃,该条件下白芨多糖提取率为40.99%,最优方案的白芨多糖溶液呈现“非牛顿流体”特征,加热温度和放置时间与黏度呈负相关,具有较好的耐盐性,该研究结果可为白芨多糖产业化的开发奠定理论基础。
Polysaccharide is the most abundant substance in the tuber of Bletilla striata,and water extraction and alcohol precipitation is the most simple and effective method to obtain polysaccharide from Bletilla striata.On the basis of single factor experiment,with liquid-solid ratio,extraction time and extraction temperature as the influencing factors and extraction yield as the response value,the water extraction and alcohol precipitation process of polysaccharide from Bletilla striata is optimized,and the effects of storage time,shear rate,heating temperature and salt tolerance on the viscosity of polysaccharide from Bletilla striata are investigated.The results show that the optimum extraction conditions are as follows:the solid-liquid ratio is 1∶59.77,the extraction time is 1.42 h,and the extraction temperature is 67.79℃.Under these conditions,the extraction yield of polysaccharide from Bletilla striata is 40.99%.Bletilla striata polysaccharide solution shows the characteristics of“non-Newtonian fluid”,and the heating temperature and storage time are negatively correlated with the viscosity,which shows good salt tolerance.The research results can lay a theoretical foundation for the development of Bletilla striata polysaccharide industry.
作者
王自凡
卢永仲
张振
WANG Zi-fan;LU Yong-zhong;ZHANG Zhen(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;College of Food and Pharmaceutical Engineering,Guizhou Institute of Technology,Guiyang 550003,China;Guizhou Academy of Testing and Analysis,Guiyang 550002,China)
出处
《中国调味品》
CAS
北大核心
2022年第7期58-63,共6页
China Condiment
基金
贵州省特色优势植提特征指标挖掘及产业化高质量开发研究(黔科院C字[2021]4号)
特殊医学用途配方食品技术研发创新平台建设(黔科合服企[2019]4001)。
关键词
白芨多糖
提取
流变性
响应面法
Bletilla striata polysaccharide
extraction
rheological properties
response surface method
