摘要
本研究通过对木薯根系分泌物进行提取、分离与鉴定,探究不同木薯品种根系分泌物的差异,为筛选抗化感耐连作木薯新种质提供参考。以木薯组培苗琼脂培养基为试验材料,采用正交试验设计,结合GC-MS技术考察不同萃取材料和洗脱剂对木薯根系分泌物的提取和分离效果,选择最优方案测定‘新选048’‘南植199’和‘华南205’的根系分泌物。结果表明:(1)木薯根系分泌物水溶性物质提取的最优方案为:用去离子水超声提取捣碎的琼脂培养基30 min,固液分离后用XAD-2萃取、无水乙醇洗脱,浓缩后用GC-MS检测,成功鉴定出包括有机酸类、醇类、酯类、酮类、醛类等26种有机化合物。(2)醇溶性物质提取的最优方案为:用50%乙醇超声提取捣碎的琼脂培养基30 min,固液分离后用XAD-4萃取、无水乙醇洗脱,浓缩后用GC-MS检测,鉴定出包括有机酸类、醚类、酯类、酮类、醛类等15种有机化合物。(3)不同品种根系分泌物的水溶性和醇溶性成分均有差异。‘南植199’根系分泌物的主要水溶性物质有羟乙酸甲酯(相对含量为3.72%)、羟基丙酮(2.40%)、甲肼(1.79%)等,主要醇溶性物质有乙醇醛(18.89%)、羟基丙酮(2.47%)、甲酸(2.25%)等;‘新选048’的主要水溶性物质有甲酸(2.68%)、1,5-戊二醇(2.39%)、丙烯酸羟乙酯(2.01%)等,主要醇溶性物质有乙醇醛(17.00%)、甲酸(2.62%)、羟基丙酮(2.46%)等;‘华南205’的主要水溶性物质有甲酸(2.23%)、羟基丙酮(1.80%)、羟乙酸甲酯(1.43%)等,主要醇溶性物质有乙醇醛(16.58%)、甲酸(3.06%)、八氟戊醇(2.98%)等。不同木薯品种的根系分泌物种类和含量均有差异,从而导致其抗化感耐连作能力的差异,为筛选耐连作品种缓解木薯连作障碍成为可能。
Autotoxicity caused by the auto-toxicity of root exudates has been proved as one of the most important reasons for continuous cropping obstacle.Root exudates of many plant types have been characterized.However,specific components of cassava root exudates have not been explored.The current study focused on the comparative effects for using different materials and eluants during the extraction and the separation processes of cassava root exudates.Then the best approach for extraction and characterization of the compounds of cassava root exudates were applied for analysing the root exudates of cassava varieties‘XX048’‘NZ199’and‘SC205’.The optimal protocol for extraction of water-soluble substances of cassava root exudates can be described as follows:the agar medium was extracted within distilled water for 30 minutes using ultrasonic waves,after the solid-liquid separation process,the compounds in the filtrate was adsorpted with XAD-2,then eluted by ethanol,and analyzed by the gas chromatography-mass spectrometry(GC-MS).In total,twenty-six water-soluble substances were identified.Meanwhile,the optimal protocol for extraction of ethanol-soluble substances of cassava root exudates were as follows:the agar medium was extracted within 50%ethanol for 30 minutes using ultrasonic waves,the compounds in the filtrate were adsorpted with XAD-4 and then eluted also by ethanol.Fifteen compounds were identified by GC-MS.Furthermore,the dominating water-soluble substances in root exudates of cassava variety‘NZ199’were methyl hydroxyacetate(the relative content was 3.72%),hydroxyacetone(2.40%)and methylhydrazine(1.79%),and the main alcohol-soluble substances of‘NZ199’were ethanol aldehyde(18.89%),hydroxyacetone(2.47%)and formic acid(2.25%).For‘XX048’,the main water-soluble substances were formic acid(2.68%),1,5-pentanediol(2.39%)and hydroxyethyl acrylate(2.01%),while the main alcohol-soluble substances were ethanol aldehyde(17.00%),formic acid(2.62%)and hydroxyacetone(2.46%).For‘SC205’,the main water-soluble substances were formic acid(2.23%),hydroxyacetone(1.80%)and methyl hydroxyethylate(1.43%),while the main alcohol-soluble substances were ethanol aldehyde(16.58%),formic acid(3.06%)and octafluoropentanol(2.98%).Thus,the chemical substances and the relative contents of root exudates varied with genotypes,leading to different resistant abilities of cassava varieties for continuous cropping obstacle.
作者
韩笑
杨慰贤
覃锋燕
范晓苏
黎亮武
黄凯
阳太亿
申章佑
韦茂贵
HAN Xiao;YANG Weixian;QIN Fengyan;FAN Xiaosu;LI Liangwu;HUANG Kai;YANG Taiyi;SHEN Zhangyou;WEI Maogui(College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China;Economic Crop Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530004,China;Guangxi Key Laboratory of Agro-Environment and Agro-Product Safety,Nanning,Guangxi 530004,China)
出处
《热带作物学报》
CSCD
北大核心
2022年第6期1248-1258,共11页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31960389)
广西自然科学基金项目(No.2018JJA130338)。
