摘要
研究采用丙烷、四氟乙烷、正丁烷、正丁烷与乙醇(9:1)混合物为溶剂,分别对樟树Cinnamomum camphora叶进行亚临界提取。提取得到的叶精油用GC/MS 法进行成分分析,并开展植物病原菌的抑制作用研究,以比较不同提取溶剂获得的产物在化学组成成分和抑菌效果上的差异。结果表明:(1)以正丁烷与乙醇(9:1)混合物为溶剂的亚临界萃取精油得率(3.67%)高于其他3种溶剂;(2)在4种樟树叶精油样品中鉴定出的化合物分别为29、33、34和22种,以醇类和烯烃类化合物为主,其中共同鉴定出的化合物为18种;所有样品均以龙脑为主要成分,其相对含量分别为44.91%、43.85%、45.41%和50.44%;(3)4种樟树叶精油对真菌病原菌水稻纹枯病原菌Rhizoctonia solani、辣椒疫霉病原菌Phytophthora capsici和细菌病原菌青枯菌Ralstonia salanacearum、胡萝卜软腐果胶杆菌Pectobacterium carotovorum均有不同程度的抑制作用,四氟乙烷萃取得到的樟树叶精油抑菌效果最优。
This study used four different solvents,i.e.propane,tetrafluoroethane,butane and mixture of butane with ethanol (9∶1) for subcritical extraction of essential oil from Cinnamomum camphora leaves.The chemical components of four essential oil samples were analyzed by GC/MS,and their inhibition effects against two plant pathogens were surveyed.Our results suggested:(1) Subcritical extraction using mixture of butane with ethanol (9∶1) had higher essential oil yield (3.67%) than other three solvents.(2) There were 29,33,34 and 22 compounds were identified respectively in four essential oil samples.There were 18 components were shared by all the four samples and borneol was the highest in relative content,respectively (44.91%,43.85%,45.41% and 50.44%).(3) All the four essential oil samples had inhibition effect on tested pathogens,and the essential oil extracted with tetrafluoroethane showed the best antifugus and antibacteria activities.
作者
胡珊
黄皓
陈杰连
梁卫驱
连辉明
侯晨
罗华建
谢佩吾
HU Shan;HUANG Hao;CHEN Jielian;LIANG Weiqu;LIAN Huiming;HOU Chen;LUO Huajian;XIE Peiwu(Dongguan Agricultural Science Research Institution,Dongguan,Guangdong,523086,China;Guangdong Academy of Forestry/Guangdong Provincial Key Laboratory of Silvicuture,Protection and Utilization,Guangzhou,Guangdong 510520,China)
出处
《林业与环境科学》
2022年第2期52-59,共8页
Forestry and Environmental Science
基金
广东省林业科技创新项目(2022KJCX006)
东莞市社会科技发展(重点)项目(2019507101198)。
关键词
樟树叶精油
亚临界萃取
气相色谱-质谱法
龙脑
抑菌
camphor leaves essential oil
subcritical extraction
GC/MS
borneol
antimicrobial effect
