摘要
目的探究依达拉奉在大鼠心肌缺血损伤和炎症反应中的作用。方法将24只SD大鼠随机均分为假手术组、模型组和依达拉奉组。模型组和依达拉奉组采用冠状动脉左前降支结扎法建立大鼠心肌损伤模型,尾静脉分别注射给与生理盐水、依达拉奉(每天3 mg/kg),注射14天后检测心脏功能、心肌梗死面积、心肌细胞凋亡、心肌组织炎症因子水平及核因子-κB(NF-κB)通路变化。结果与模型组比较,依达拉奉组大鼠心功能显著改善,左心室后壁舒张期末厚度减少,左心室射血分数和左心室缩短分数增加(P<0.05)。大鼠炎症因子水平、心肌梗死面积及心肌细胞凋亡数量模型组较假手术组显著升高,依达拉奉组较模型组显著降低(P<0.05)。心肌组织p-NF-κB抑制剂激酶α/β、p-p65、p65水平模型组较假手术组显著升高(P<0.05),依达拉奉组较模型组显著降低(P<0.05)。结论依达拉奉能够改善大鼠心肌缺血损伤和炎症反应。
Aim To investigate the effects of edaravone on myocardial ischemia and inflammation in rats.Methods 24 SD rats were randomly divided into sham operation group,model group and edaravone group.Myocardial injury model was established by ligation of left anterior descending coronary artery,and edaravone was injected into tail vein(3 mg/kg per day).Cardiac function,myocardial infarction size,myocardial apoptosis,inflammatory factors in myocardial tissue and nuclear factor kappa-B(NF-κB)pathway were measured 14 days after injection.Results Compared with model group,edaravone group significantly improved cardiac function,left ventricular posterior wall diastolic thickness decreased,left ventricular ejection fraction and left ventricle shortens fraction increased(P<0.05).The levels of inflammatory factors,myocardial infarction area and myocardial cell apoptosis in model group were significantly increased compared with sham group,and edaravone group was significantly decreased compared with model group(P<0.05).The levels of P-IKKα/β,p-p65 and P65 in myocardial tissue of model group were significantly increased compared with sham group(P<0.05),while those in edaravone group were significantly decreased compared with model group(P<0.05).Conclusion Edaravone can improve myocardial ischemia injury and inflammatory response in rats.
作者
王娟
顾顺忠
陆洋
颜永进
WANG Juan;GU Shunzhong;LU Yang;YAN Yongjin(Department of Cardiology,Hai'an People's Hospital,Hai'an 226600,Jiangsu,China)
出处
《中南医学科学杂志》
CAS
2022年第4期495-498,共4页
Medical Science Journal of Central South China
基金
江苏省卫生计生委科研项目(LGY201704)。
