摘要
目的研究通微消痔颗粒的药效学作用。方法(1)采用试管内稀释法观察通微消痔颗粒对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、枯草芽孢杆菌、白色念珠菌的体外抑菌作用。(2)通过巴豆油致小鼠耳郭肿胀实验、鸡蛋清致大鼠足趾肿胀,建立巴豆油致大鼠肛门肿胀模型,并检测血清白细胞介素6(IL-6)及肿瘤坏死因子α(TNF-α)水平,观察通微消痔颗粒的抗炎作用。(3)通过热板实验和扭体实验,观察通微消痔颗粒的镇痛作用。(4)通过玻片法凝血功能评估实验、剪尾法出血时间评估实验,观察通微消痔颗粒的止血作用。结果(1)通微消痔颗粒对5种菌株均具有体外抗菌作用,其中对白色念珠菌的抗菌效果最好。(2)通微消痔颗粒可抑制巴豆油所致小鼠耳郭肿胀(肿胀抑制率为7.24%~19.00%),可减轻大鼠足趾肿胀度,降低直肠肛门系数、血清IL-6及TNF-α水平(均P<0.05)。(3)通微消痔颗粒可提高小鼠痛阈值,减少小鼠扭体次数,但与正常小鼠相比差异无统计学意义(P>0.05)。(4)通微消痔颗粒可缩短小鼠出血时间(P<0.05)和凝血时间。结论通微消痔颗粒具有抗菌、消炎、镇痛、止血、改善小肠运动的功能,对痔疮有很好的治疗作用。
Objective To study the pharmacodynamic effects of Tongwei Xiaozhi Granules.Methods(1)The tube dilution method was used to observe the antibacterial effects in vitro of Tongwei Xiaozhi Granules on Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Bacillus subtilis,and Candida albicans.(2)The rat model of anus swelling caused by croton oil was established through the croton oil-caused mouse ear swelling test and rat′s paw edema caused by egg white.Serum interleukin 6(IL-6)and tumor necrosis factorα(TNF-α)levels were detected,and the anti-inflammatory effects of Tongwei Xiaozhi Granules were observed.(3)The analgesic effects of Tongwei Xiaozhi Granules were observed by the hot plate test and the writhing test.(4)The hemostasis effects of Tongwei Xiaozhi Granules were observed by the coagulation function assessment experiment using the slide test and the bleeding time assessment experiment using the tail-cutting test.Results(1)Tongwei Xiaozhi Granules had antibacterial effects in vitro on the five strains,among which Candida albicans yielded the best antibacterial effect.(2)Tongwei Xiaozhi Granules could inhibit mouse ear swelling caused by croton oil(with a swelling inhibitory rate ranged from 7.24%to 19.00%),and reduce rat paw edema degree,anorectum index,and serum IL-6 and TNF-αlevels(all P<0.05).(3)Tongwei Xiaozhi Granules could improve mouse′s pain threshold value and reduce the number of mouse writhing,but did not statistically differ from the control group(P>0.05).(4)Tongwei Xiaozhi Granules could shorten mouse′s bleeding time(P<0.05)and coagulation time.Conclusion Tongwei Xiaozhi Granules has antibacterial,anti-inflammatory,analgesic and hemostatic functions,along with the function of improving small intestinal movement,exerting an excellent treatment effect on hemorrhoids.
作者
李开双
李燕婧
苏华
曾宪彪
邓聿胤
郑海平
LI Kai-shuang;LI Yan-jing;SU Hua;ZENG Xian-biao;DENG Yu-ying;ZHENG Hai-ping(Institute of Traditional Chinese Medicine Pharmacology,Guangxi Institute of Chinese medicine&Pharmaceutical Science,Nanning 530022,China)
出处
《广西医学》
CAS
2022年第9期970-977,共8页
Guangxi Medical Journal
基金
广西科技计划(桂科AB18221098)。
关键词
痔疮
通微消痔颗粒
药效学
动物实验
Hemorrhoids
Tongwei Xiaozhi Granules
Pharmacodynamics
Animal experiment
