摘要
水稻米糠油中富含的甾醇阿魏酸酯(Steryl ferulate)是一类重要的含脂的生物活性化合物,不仅影响种子发育、干旱胁迫响应和膜脂代谢等生物学过程,也直接影响着稻米营养品质和人类健康。尽管如此,目前,我们对水稻甾醇阿魏酸酯的组成及其代谢通路知之甚少。拟南芥酰基辅酶A甾醇酰基转移酶1 (AtASAT1)是拟南芥膜结合O-酰基转移酶基因家族中唯一具有酰基辅酶A甾醇酰基转移酶(ASAT)活性的成员。该酶以饱和脂肪酰基辅酶A为酰基供体,环芳烃为酰基受体。过表达AtASAT1能显著增加植物种子中甾醇酯的积累。本研究通过生物信息学的分析方法,从公共数据库中鉴定筛选出包括拟南芥和番茄在内的10种植物105个ASAT家族成员,构建ASAT蛋白家族进化树,对比该家族成员在油料物种和非油料物种、单子叶植物和双子叶植物间进化关系与特征。同时对筛选出的14个水稻ASAT蛋白(OsASAT)进行蛋白结构、理化性质分析,对Os ASAT基因家族进行染色体分布、基因结构、启动子序列、表达模式等分析。本研究结果为进一步研究ASAT蛋白生物学功能及其在米糠油合成中的作用提供了理论依据。
Rice bran oil is rich in bioactive steryl ferulates that are important not only for seed development,drought stress and lipid metabolism, but also for grain nutrition quality and human healthy. However, its complete compositions and associated metabolic pathways are poorly understood. Arabidopsis thaliana Acyl-CoA Sterol Acyltransferase1(AtASAT1) is the only one in the superfamily of Membrane-Bound O-Acyltransferases that has ASAT activity, which prefers saturated fatty acyl-CoAs as acyl donors and cycloartenol as the acyl acceptor. Overexpression of AtASAT1 in Arabidopsis results in a strong accumulation of cycloartenol fatty acyl esters accompanied by an increase of the whole steryl esters contents in seeds. Here we present our bioinformatic based analysis data aiming to identify more plant ASAT family members and to in silico study of them in rice. We identified 105 ASAT Family members from public genome database of 10 plant species including Arabidopsis and tomato, and the phylogenetic tree of ASAT family constructed revealed evolutionary relationships of ASAT family members between oil crops and non-oil crops, and between monocotyledons and dicotyledons. We further investigated into physical and chemical properties of 14 Os ASAT proteins, and chromosome distributions, structures and promoter sequences, and expression patterns of corresponding OsASAT genes, providing a solid theoretical basis for further study of the biological functions of Os ASAT proteins.
作者
孙文礼
石建新
Sun Wenli;Shi Jianxin(Joint International Research Laboratory of Metabolic&Developmental Sciences,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai,200240)
出处
《分子植物育种》
CAS
北大核心
2022年第11期3481-3498,共18页
Molecular Plant Breeding
基金
国家自然科学基金项目(31971907)
高等学校学科创新引智计划项目(111项目,B14016)共同资助
关键词
甾醇酯化
ASAT
蛋白结构
分子进化
表达模式
Sterol esterification
ASAT
Protein structure
Molecular evolution
Expression pattern
