摘要
采用iTRAQ及生物信息分析技术研究三角帆蚌珍珠囊及外套膜外膜组织蛋白组学差异,共鉴定出1871个蛋白,其中显著性差异表达蛋白119个(P<0.05),包括上调蛋白74个,下调蛋白45个,与免疫相关的蛋白有37个,其中19个上调蛋白,18个为下调蛋白,主要富集于Notch、Hippo、NF-κB、TGF-β等信号通路,参与免疫球蛋白、凋亡、损伤愈合以及炎症反应。上调蛋白中的MRC1、LBP、EPX、FcGBP以及下调蛋白中的CD109、CNN3、NOTCH3、LAMB2等在外套膜插核后5和20 d的插核组织及50和90 d的珍珠囊组织的蛋白水平和mRNA表达水平趋势相同,推测珍珠囊形成过程中贝类通过这些差异表达蛋白调节细胞免疫以及炎性反应,并促进移植小片和外套膜愈合形成珍珠囊的过程。研究结果为理解三角帆蚌插核引起的免疫提供了蛋白水平的基础资料。
A total of 1871 proteins were identified by iTRAQ and bioinformatics analysis,among which 119 differentially expressed proteins(P<0.05),including 74 up-regulated proteins and 45 down-regulated proteins.And 37 immune-related proteins were screened,including 19 up-regulated proteins and 18 down-regulated proteins,which are mainly enriched in Notch,Hippo,NF-κB,TGF-βand some other signaling pathways which related to the immunoglobulin,wound healing and inflammation.Up-regulated proteins such as MRC1 LBP,EPX,FCGBP,and down-regulated proteins like CD109,CNN3,NOTCH3,LAMB2 had the similar trend as the mRNA expression level in tissues 5,20,50,90 days after inserting.It is speculated that in the process of pearl sac formation,molluscs may protect cells from the damage by immune mechanism and inflammation through these differential proteins,and promote the fusion of pearl sac and outer mantle.This study provided basic data for understanding the immune response caused by Hyriopsis cumingii nucleus insertion.
作者
李文娟
杨婧漪
冯上乐
李雪男
陈一格
申晓雅
白志毅
LI Wenjuan;YANG Jingyi;FENG Shangle;LI Xuenan;CHEN Yige;SHEN Xiaoya;BAI Zhiyi(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2022年第2期344-354,共11页
Journal of Shanghai Ocean University
基金
国家重点研发计划(2018YFD0901406)
上海市优秀学术带头人计划(19XD1421500)。
