摘要
黄瓜枯萎病(Cucumber Fusarium wilt)是黄瓜生产上发生最普遍的病害之一,严重时甚至造成绝产,目前仍没有有效环保的防治方法。本试验利用西芹腐根丙酮两次层析物作用于黄瓜枯萎病菌,通过化感作用效果筛选出4个最佳流分,将最佳流分作为诱导剂灌根诱导处理黄瓜幼苗,并人工接种黄瓜枯萎病菌,观测比较不同处理对黄瓜枯萎病的诱导抗性;之后选取最强诱导剂诱导处理后且未接病菌的黄瓜幼苗进行转录组学分析。结果表明,4个最佳流分为RRA32、RRA38、RRA101和RRA102,对黄瓜枯萎病的诱导抗性效果分别为65.85%~78.95%、68.29%~81.58%、77.5%~86.84%和82.5%~89.47%,与对照差异极显著,比较确定RRA102为最强诱导剂。对流分RRA102诱导处理后的黄瓜幼苗进行转录组学分析,共获得差异基因322个,其中上调表达152个,下调表达170个。差异基因中228个获得GO数据库功能注释,在Level 1水平上发现,差异基因主要富集在过氧化氢的反应和碳酸盐脱水酶活性条目上,在Level 2水平上分析发现,差异基因主要富集在免疫系统过程、抗氧化活性和电子载体活性等条目上;KEGG数据库富集分析发现,共103个基因被注释到63个通路中,显著富集在氮代谢、抗坏血酸和醛酸代谢、氨基酸的生物合成、氨基糖和核苷酸糖代谢和植物激素信号转导代谢通路中,以上通路均与植物抗病性有关,说明黄瓜幼苗在诱导处理后激发了自身的防御系统,进而有效抑制黄瓜枯萎病的发生,为进一步更绿色有效的防控黄瓜枯萎病,挖掘抗病基因提供理论基础。
Cucumber Fusarium wilt is one of the most common diseases in cucumber production,which even leads to death of plants.At present,there is still no effective control method in an environment-friendly way.In this study,the fractions of the column chromatography of acetone extract from celery rotten roots(twice cycle)were used to act on Fusarium oxysporum f.sp.cucumerinum(FOC).According the allelopathic inhibition effect,the four best fractions RRA32,RRA38,RRA101 and RRA102 were obtained as inducers to treat cucumber seedlings by root irrigation.After induction,the seedlings were challenge-inoculated with FOC and the control efficiencies were 65.85%-78.95%,68.29%-81.58%,77.5%-86.84%and 82.5%-89.47%,respectively,which showed significantly inhibitory than blank and acetone control.The fresh cucumber seedlings were then treated with the strongest inducer RRA102 for one week with water treatment as control.The fresh leaves were flash frozen in liquid nitrogen and sent for RNA sequencing analysis.Through differential expression analysis of RNA-seq performed by Illumina NovaSeq 6000,a total of 322 differentially expressed genes(DEGs)were identified,of which 152 were up-regulated and 170 were down-regulated.With GO function annotation classifications,a total of 228 genes were divided into different function categories,which mainly enriched in response to hydrogen peroxide and carbonate dehydratase activity or in immune system process,antioxidant activity and electron carrier activity and other items via the way of clustering levels.KEGG enrichment analysis showed that a total of 103 genes were annotated to 63 metabolic pathways with nitrogen metabolism,ascorbic acid and aldonic acid metabolism,amino acid biosynthesis,amino sugar and nucleotide sugar metabolism,and plant hormone signaling pathway significantly enriched,which were related to plant disease resistance.The transcriptome of cucumber seedlings after induction treatment exhibited that their own defense system stimulated,thereby,might effectively inhibit the occurrence of cucumber Fusarium wilt.This study provided a theoretical basis for further green and effective prevention and control of cucumber Fusarium wilt,and identifying the resistant genes.
作者
郭嘉华
武兆昕
李蕾
云兴福
GUO Jiahua;WU Zhaoxin;LI Lei;YUN Xingfu(Inner Mongolia Agricultural University,College of Horticulture and Plant Protection,Hohhot 010010,China;Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031,China;Manzhouli College of Inner Mongolia University,Manzhouli Russian Vocational and Technical College,Manzhouli 021400,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2022年第3期364-376,共13页
Acta Phytopathologica Sinica
基金
国家自然科学基金项目(31160100)
内蒙古自治区科技成果转化项目(CGZH2018140)
内蒙古自治区应用技术研究与开发重点项目(201702068)。
关键词
西芹腐根
丙酮浸提液
柱层析
黄瓜枯萎病
诱导抗性
转录组学
Celery rotten root
Acetone extract
Column chromatography
Cucumber Fusarium wilt
Induced resistance
Transcriptome analysis
