摘要
目的研究中药制剂牛黄清感胶囊对新型冠状病毒的抑制作用,可为药物筛选和新冠肺炎治疗提供实验依据。方法用Vero-E6细胞分离和培养新型冠状病毒GS048株,制备的牛黄清感胶囊溶液体外与新冠病毒共孵育后测定病毒滴度改变;将含有药物的培养液作用于新型冠状病毒接种的细胞,测定牛黄清感胶囊溶液在细胞培养中对新型冠状病毒增殖的抑制作用。结果实验发现药物浓度低于274.3μg/ml时对细胞无毒害作用,药物和新型冠状病毒共孵育后可使病毒滴度从10^(4.4)TCID_(50)/ml下降至10^(2.8)TCID_(50)/ml;体外新冠病毒细胞培养实验中,加入药物使细胞病变数减少,病毒滴度对数值下降了40%~50%。结论牛黄清感胶囊在体外具有部分抑制新冠病毒的能力,可作为候选药物进一步验证对新冠肺炎的治疗作用。
Objective Objective The inhibitory effect of Niuhuang Qinggan Capsule on 2019-nCoV virus was studied to provide experimental evidence for drug screening and treatment of 2019-nCoV acute respiratory disease.Methods The 2019-nCoV virus GS048 strain was isolated and cultured by Vero-E6 cells.The solution of Niuhuang Qinggan capsule was incubated with virus in vitro to determine the change of virus titer.The effect of Niuhuang Qinggan capsule on the proliferation of 2019-nCoV virus was determined by cell culture medium method.Results It was found that the concentration of 274.3μg/ml was not toxic to the cells when the concentration of drugs was lower.When the drug was incubated with virus,the titer of virus decreased from 10^(4.4)TCID_(50)/ml to 10^(2.8)TCID_(50)/ml.In vitro 2019-nCoV virus culture experiment showed that the addition of the drugs reduced the cytopathy,and the logarithmic value of virus titer decreased by 40%-50%.Conclusion In this study,in vitro inhibition experiments suggest that Niuhuang Qinggan capsule has the potential to inhibit the proliferation of 2019-nCoV.It can be used as a candidate drug to further verify the therapeutic effect of the 2019-nCoV acute respiratory disease.
作者
卢学新
祝双利
刘亚宁
赵雪莹
遆铁军
周有财
Lu Xuexin;Zhu Shuangli;Liu Yaning;Zhao Xueying;Di Tiejun;Zhou Youcai(NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Heilongjiang University of China Medicine,Harbin 150040,China;Heilongjiang Aolidanede Pharmaceutical Co.,Ltd,Suihua 152400,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2022年第2期128-130,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家重点研发计划(2019YFC1200701)。
关键词
牛黄清感胶囊
新冠病毒
新冠肺炎
治疗
药物筛选
Niuhuang Qinggan Capsule
2019 novel coronavirus
Coronavirus pneumonia
Pneumonia treatment
Drug screening
