摘要
收获指数是一个重要的农艺性状,甘蓝型油菜的收获指数偏低,有较大的改良空间,研究油菜收获指数的遗传机理对该性状的改良具有重要的指导意义。本研究利用已构建的高密度遗传图谱对由高、低收获指数亲本衍生而来的包含186个株系的重组自交系进行收获指数的数量性状位点(quantitative trait locus,QTL)定位,2016—2018连续3年环境及最佳线性无偏预测(best linear unbiased prediction,BLUP)值共定位到12个收获指数相关QTL,分别位于A03、A05、A06、A07、A09、C04和C05_random染色体上,单个QTL解释的表型变异在1.27%~14.20%之间。同时,利用588份重测序自然群体对收获指数性状开展了全基因组关联分析(genome-wide association study,GWAS),2016、2017、2019年及BLUP值共检测到6个显著关联位点,分别位于A09、C01和C03染色体上,其中2019年环境检测到的位于A09上的S9_25882060与S9_25961704重叠,且与2016年环境检测到的位于A09染色体上的S9_24834640位置接近,此外2016年环境下GWAS检测到的位点2016HI(S9_24834640)位于2017年环境下重组自交系群体定位的q2017HI-9区段内。对比前人研究,本研究获得的18个位点中有1个位点与收获指数相关位点重叠,有6个位点与产量相关性状位点接近。结合本课题组已有的转录组测序结果,在QTL区段及显著关联的SNP位点附近筛选出36个重点候选基因,这些基因主要涉及光合作用、跨膜运输、储藏物质合成及转录调控等。这些研究结果为甘蓝型油菜收获指数的遗传改良提供了重要的理论依据。
Harvest index(HI)is an important agronomic trait,and the HI of rapeseed(Brassica napus L.)remains much lowerer than that of other major crops,indicating there is large scope for improvement of this trait.It is important to explore the genetic mechanism of rapeseed harvest index for its genetic improvement.In this study,QTLs for HI using the 186 recombinant inbred lines(RIL)in the three environments(2016,2017,and 2018)and best linear unbiased prediction(BLUP)were detected based on the high-density genetic linkage map.A total of 12 HI QTLs located on chromosomes A03,A05,A06,A07,A09,C04,and C05_random were detected,with the explained phenotypic variation of individual QTL range from 1.27%to 14.20%.GWAS for HI was performed using 588 resequencing natural populations established by our previous study.A total of 6 SNPs associated with HI were detected in three years(2016,2017,and 2019)and BLUP,which located on chromosomes A09,C01,and C03.The S9_25882060 on chromosome A09 detected by the environment in 2019 overlapped with S9_25961704,and was close to S9_24834640 on chromosome A09 detected by the environment in 2016.In addition,2016HI(S9_24834640)detected in 2016 was located in the interval region of q2017HI-9.Among these 18 loci,one locus was overlapped with reported HI-related QTLs,and six loci were contiguous to reported yield-related QTLs.Combining the results of transcriptome sequencing data of our labo-ratory,36 primary candidate genes were selected and these genes involved in the photosynthesis,transmembrane transport,storage components synthesis,transcriptional regulation and so on.These results provide important theoretical basis for genetic improve-ment of HI in rapeseed.
作者
张超
杨博
张立源
肖忠春
刘景森
马晋齐
卢坤
李加纳
ZHANG Chao;YANG Bo;ZHANG Li-Yuan;XIAO Zhong-Chun;LIU Jing-Sen;MA Jin-Qi;LU Kun;LI Jia-Na(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China;Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China;Oil Research Institute of Guizhou Province,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China)
出处
《作物学报》
CAS
CSCD
北大核心
2022年第9期2180-2195,共16页
Acta Agronomica Sinica
基金
国家重点研发计划项目(2018YFD0100500)
国家自然科学基金项目(31830067,31871653)
高等学校学科创新引智基地项目“111”工程项目(B12006)
重庆市研究生科研创新项目(CYB20102,CYS20124)资助。
