摘要
【目的】建立华重楼组培快繁技术体系。【方法】以华重楼幼苗为试材,研究外植体预处理方式和消毒方法,筛选适宜诱导的外植体,筛选不定芽诱导、增殖以及生根和结鳞茎最佳培养基。【结果】华重楼外植体先经过0.5%多菌灵浸泡2 h,再利用75%乙醇浸泡45 s和0.1% HgCl;消毒10 min,污染率最低(1.67%),诱导的不定芽长势最好。高5~10 cm、带根系的华重楼幼苗或去掉茎叶的幼苗是最适外植体,不定芽诱导最佳培养基为WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L蔗糖,诱导率达98.33%,萌发的新芽生长更快;不定芽增殖最适培养基为MS+2.0 mg/L 6-BA+1.0 mg/L IAA+30 g/L蔗糖,培养90 d后增殖系数为3.75;不定芽生根和结鳞茎最适培养基为WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L蔗糖,生根和结鳞茎率达96.67%,根系数3.84条,根长4.15 cm,鳞茎直径0.86 cm。生根苗3—5月份移栽到基质为泥炭土︰珍珠岩︰河沙=2∶1∶1的组合物中成活率最高,为30%。【结论】建立了适合华重楼幼苗组培快繁的技术体系,为华重楼的资源保护及规模化生产优质种苗奠定了基础。
【Objective】The study was conducted to establish a tissue culture and rapid propagation system for Paris polyphylla var.chinensis.【Method】The seedlings of P.polyphylla var.chinensis were selected as materials in this experiment.Different pretreatment and sterilization method for explants were studied.And the optimum explants,the suitable medium for adventitious buds induction,propagation,rooting and bulb setting were also screened.【Result】The contamination rate reached the lowest (1.67%) and the new buds grew best after the explants was dipped into 0.5% carbendazim for 2 h and then dipped into 75% ethanol for 45 s,and 0.1% HgCl;for 10 min.The best medium for adventitious buds induction was WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose,the induction rate reached up to 98.33% and new buds grew fast and well when the seedlings or the seedlings without leaves and stems were selected as explants,which were 5-10 cm high with roots.The optimal medium for adventitious buds propagation was MS + 2.0 mg/L 6-BA+1.0 mg/L IAA+30 g/L sucrose and the propagation coefficient reached 3.75 times after being cultured for 90 d.While the optimal medium for adventitious buds rooting and bulb culture was WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose,the rooting and bulb setting rate reached 96.67%,the root number was 3.84,the root length was 4.15 cm and the bulb diameter was 0.86 cm.The best medium for transplanting and domestication of seedlings was peat soil∶perlite∶river sand=2∶1∶1 on March to April and the survival rate reached 30%.【Conclusion】A tissue culture and rapid propagation system for P.polyphylla var.chinensis seedlings was established,which laid a foundation for resource conservation and mass production of seedlings of P. polyphylla var. chinensis.
作者
吴巧芬
夏科
赵志国
马晓雅
仇硕
WU Qiaofen;XIA Ke;ZHAO Zhiguo;MA Xiaoya;QIU Shuo(Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization/Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China;College of Tourism&Landscape Architecture,Guilin University of Technology,Guilin 541006,China)
出处
《广东农业科学》
CAS
2022年第5期10-18,共9页
Guangdong Agricultural Sciences
基金
广西重点研发计划项目(桂科AB18294026)
广西科学院基本业务费项目(2018YJJ909)
广西科学院园艺植物种质资源创新及利用创新团队启动经费项目(CQZ-E-1919)
广西植物研究所基本业务费项目(桂植业22006)。
关键词
华重楼
消毒
不定芽
诱导
增殖
生根和结鳞茎
Paris polyphylla var.chinensis
sterilization
adventitious bud
induction
propagation
rooting and bulb setting
