摘要
开发一种高效、可同时测定7种紫杉烷类化合物含量的高效液相色谱(HPLC)检测方法,对3种红豆杉属植物中7种紫杉烷类化合物的含量进行比较分析。HPLC检测条件为:色谱柱为HiQsil C_(18)色谱柱;流动相为乙腈(A)-水(B);梯度洗脱程序为①0~10 min,30%A,②10~12 min,30%~45%A,③12~25 min,45%A,④25~30 min,42%~45%A,⑤30~40 min,30%~42%A;检测波长为227 nm。对各紫杉烷类化合物成分质量分数和峰面积进行线性回归,并考察不同添加量下7种紫杉烷类化合物的加样回收率。结果表明,东北红豆杉、南方红豆杉、曼地亚红豆杉树皮中紫杉醇含量分别为0.234、0.043、0.167 mg/g,三尖杉宁碱含量分别为0.092、0.034、0.053 mg/g,10-去乙酰基巴卡亭含量分别为0.376、0.158、0.063 mg/g,10-去乙酰基紫杉醇含量分别为0.157、0.113、0.126 mg/g,巴卡亭Ⅲ含量分别为0.395、0.116、0.074 mg/g,7-表-10-去乙酰基紫杉醇含量分别为0.053、0.027、0.033 mg/g,7-木糖-10-去乙酰基紫杉醇量分别为0.266、0.564、0.315 mg/g。东北红豆杉中紫杉烷类化合物含量最多,其次是南方红豆杉,在曼地亚红豆杉中含量最低。7种紫杉烷类化合物在所测定的线性范围内均具有良好的线性关系,加样回收率试验结果表明该方法回收率较高。应用建立的HPLC分析方法可同时测定红豆杉属植物中7种紫杉烷类化合物的含量,该研究为红豆杉属植物的质量控制、种源选育及资源开发提供了依据。
The study was conducted to develop an efficient high performance liquid chromatography(HPLC)method for simultaneous determination of seven taxanes in Taxus,the contents of seven taxanes in three Taxus species were compared and analyzed.HPLC detection conditions were:the chromatographic column was HiQsil C_(18) column;the binary mobile phases was acetonitrile(A)-water(B);the gradient elution procedure was(0~10 min,30%A;10~12 min,30%~45%A;12~25 min,45%A;25~30 min,42%~45%A;30~40 min,30%~42%A);the detection wavelength was 227 nm.Linear regression was carried out for the mass concentration and peak area of each taxane component,and the sample recovery of 7 taxanes at different addition levels was investigated.The results showed that the contents of paclitaxel in the bark of Taxus cuspidata,Taxus chinensis var.mairei and Taxus×media cv.hicksii were 0.234,0.043,0.167 mg/g,the contents of Taxol B were 0.092,0.034,0.053 mg/g,the contents of 10-DAB were 0.376,0.158,0.063 mg/g,the contents of 10-DAT were 0.157,0.113,0.126 mg/g,the contents of BaccatinⅢwere 0.395,0.116,0.074 mg/g,the contents of 7-epi-10-DAT were 0.053,0.027,0.033 mg/g,the contents of 7-xyl-10-DAT were 0.266,0.564,0.315 mg/g.Taxanes in Taxus cuspidata were the highest,followed by Taxus cuspidate,and the lowest in Taxus×media cv.hicksii.The seven taxane compounds had good linear relationship within the linear range determined,and the test results of sample addition recovery rate showed that the recovery rate of this method was high.The established HPLC analysis method can simultaneously determine the contents of seven taxanes in Taxus,and this study provides a basis for the quality control,provenance breeding and resource development of Taxus.
作者
崔海超
郑文艺
张智慧
傅岳峰
李欣悦
付玉杰
顾成波
CUI Haichao;ZHENG Wenyin;ZHANG Zhihui;FU Yuefeng;LI Xinyue;FU Yujie;GU Chengbo(Key Laboratory of Forest Plant Ecology,Ministry of Education,Engineering Research Center of Forest Bio-Preparation,Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances,Northeast Forestry University,Harbin 150040,China;The College of Forestry,Beijing Forestry University,Beijing 100083,China)
出处
《森林工程》
北大核心
2022年第4期118-124,共7页
Forest Engineering
基金
国家自然科学基金重点项目(31930076)
黑龙江省自然科学基金项目(LH2021C015)
黑龙江头雁创新团队计划(林木遗传育种创新研究团队)。
