摘要
背景:具有生物学功能的骨修复材料一直是生物医学工程领域的研究热点。目的:从分子结构仿生与生物功能仿生的需求出发,研制具有仿生空间结构和仿生功能的仿生骨基质材料。方法:以氨基封端的聚乙二醇和均苯四甲酸酐为反应物,通过调节丁二胺加入的量(15,30,45,60μL),获得4种性状的聚乙二醇衍生物水凝胶,向4种水凝胶中分别加入基于聚乳酸和力生长因子的生物活性物质(MGF-Ct24E-MPLA-EG-g-HAP),采用溶剂共混法制备生物活性仿生骨基质材料(PAPI-BDA/MGF-Ct24E-MPLA-EG-g-HAP)。表征生物活性仿生骨基质材料的生物活性物质负载率、孔隙率、表面形貌、亲/疏水性能、吸水率及微观形貌。结果与结论:①在仿生骨基质材料制作过程中,在pH=5.8的PBS中,随着丁二胺加入量的增加,仿生骨基质材料中生物活性物质负载量呈先增多再减少的现象,当丁二胺加入量为30μL时生物活性物质负载量最高;在pH=3.5-7.4的PBS中,仿生骨基质材料(丁二胺加入量为30μL)中生物活性物质负载量呈减少趋势,当pH值增加到9.8时,仿生骨基质材料中生物活性物质负载量基本保持稳定;②在相同pH值的PBS中,随着丁二胺加入量的增加,仿生骨基质材料的孔隙率降低;当丁二胺加入量相同时,随着PBS pH值的增大,仿生骨基质材料的孔隙率呈先降低后升高的趋势;③在pH=5.8的PBS中,随着丁二胺加入量的增加,仿生骨基质材料的静态接触角减小,吸水率增加;④扫描电镜下可见,仿生骨基质材料呈连续开放孔状结构,孔间相互贯通,孔径分布230-690 nm,且孔径大小与丁二胺加入量呈反向相关,其中丁二胺加入量为30μL制备的仿生骨基质材料类似天然骨基质结构。
BACKGROUND:Bone repair materials with biological functions have always been a research focus in biomedical engineering.OBJECTIVE:To develop bionic bone matrix material with bionic space structure and bionic function based on the requirements of molecular structure biomimetic and biological function biomimetic.METHODS:Using amino-terminated polyethylene glycol and pyromellitic anhydride as reactants,four kinds of polyethylene glycol derivative hydrogels were obtained by adjusting the amount of butanediamine added(15,30,45,60μL).Bioactive substances based on polylactic acid and force growth factor(MGF-Ct24E-MPLA-EG-g-HAP)were added to the four hydrogels separately.The solvent method was used to prepare bioactive biomimetic bone matrix material(PAPI-BDA/MGF-Ct24E-MPLA-EG-g-HAP).The bioactive material loading rate,porosity,surface morphology,hydrophilic/hydrophobic properties,water absorption and microscopic morphology of the bioactive bionic bone matrix materials were characterized.RESULTS AND CONCLUSION:(1)In the production process of the bionic bone matrix material,in PBS with pH=5.8,with the increased amount of butanediamine added,the loading of bioactive substances in the bionic bone matrix material first increased and then decreased.The loading of bioactive substances was the highest when the amount was 30μL;in PBS with pH=3.5-7.4,the loading of bioactive substances in the biomimetic bone matrix material(the addition amount of butanediamine was 30μL)showed a decreasing trend.When the pH value increased to 9.8,the loading of bioactive substances in the bionic bone matrix material remained basically stable.(2)In PBS with the same pH value,the porosity of the bionic bone matrix material decreased with the increase of the addition amount of butanediamine.When the addition amount of butanediamine was the same,with the increase of the pH value of PBS,the porosity of the bionic bone matrix material first decreased and then increased.(3)In PBS with pH=5.8,with the increase of the addition of butanediamine,the static contact angle of the bionic bone matrix material decreased and the water absorption increased.(4)It can be seen under the scanning electron microscope,the bionic bone matrix material has a continuous open pore structure,the pores are interconnected,the pore size distribution is 230-690 nm,and the pore size is inversely related to the amount of butanediamine added.The bionic bone matrix material prepared by adding butanediamine in an amount of 30μL is similar to the structure of natural bone matrix.
作者
彭坤
Peng Kun(Chongqing Medical and Pharmaceutical College,Chongqing 401331,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第12期1811-1816,共6页
Chinese Journal of Tissue Engineering Research
基金
重庆市自然科学基金(cstc2018jcyjAX0828),项目负责人:彭坤
第三批重庆市高等学校青年骨干教师资助计划(渝教人发[2016]41号),项目负责人:彭坤。
关键词
聚乙二醇衍生物
丁二胺
水凝胶
聚乳酸
力生长因子E肽
仿生骨基质材料
polyethylene glycol derivatives
butanediamine
hydrogel
polylactic acid
force growth factor E peptide
bionic bone matrix material
